Human Anti-Toxin Recombinant Antibody (clone LR)
CAT#: HPAB-S0146-YC
Clone LR was obtained after three cycles of directed evolution of the parental clone 3F and incorporating a V101F mutation into one of the derived variant clones (9004G). This antibody clone recognizes and neutralizes the main toxins from the venoms of the C. noxius and Centruroides suffusus suffusus scorpions (Cn2 and Css2, respectively), with affinities in the picomolar range, and removes any poisoning symptoms. In neutralization tests using fresh whole venom in mice, clone LR was capable of inhibiting nearly all symptoms caused by the C. suffusus suffusus venom.
Published Data
Figure 1 Competition binding assays of scFvs LR and RU1 with the Cn2 toxin.
These results clearly indicated that the scFvs recognized different sites on the toxin. This is significant because the toxins were neutralized through the binding of the scFvs to non-overlapping sites, which improved their efficiency as an antivenom.
Riaño-Umbarila, L., Ledezma-Candanoza, L. M., Serrano-Posada, H., Fernández-Taboada, G., Olamendi-Portugal, T., Rojas-Trejo, S., ... & Becerril, B. (2016). Optimal neutralization of Centruroides noxius venom is understood through a structural complex between two antibody fragments and the Cn2 toxin. Journal of Biological Chemistry, 291(4), 1619-1630.
Figure 2 scFv recognition to Cn2 upon treatment under strong denaturing conditions.
ScFv LR exhibited the best recognition, followed by scFv 6009F, and finally by scFv 9004G.
Riaño-Umbarila, L., Contreras-Ferrat, G., Olamendi-Portugal, T., Morelos-Juárez, C., Corzo, G., Possani, L. D., & Becerril, B. (2011). Exploiting cross-reactivity to neutralize two different scorpion venoms with one single chain antibody fragment. Journal of Biological Chemistry, 286(8), 6143-6151.
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Centruroides noxius
- Species Reactivity
- C. noxius, C. suffusus suffusus scorpions
- Clone
- LR
- Applications
- Neut, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0934-FY | Llama Anti-Toxin Recombinant Antibody; VHH Fragment (HPAB-0934-FY) | ELISA, Neut, WB | Llama VHH |
| HPAB-0935-FY | Llama Anti-Toxin Recombinant Antibody; VHH Fragment (HPAB-0935-FY) | ELISA, Neut, WB | Llama VHH |
| HPAB-0936-FY | Llama Anti-Toxin Recombinant Antibody; VHH Fragment (HPAB-0936-FY) | ELISA, Neut, WB | Llama VHH |
| HPAB-0937-FY | Llama Anti-Toxin Recombinant Antibody; VHH Fragment (HPAB-0937-FY) | ELISA, Neut | Llama VHH |
| HPAB-0938-FY | Llama Anti-Toxin Recombinant Antibody; VHH Fragment (HPAB-0938-FY) | ELISA, Neut, WB | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0145-YC-F(E) | Human Anti-Toxin Recombinant Antibody (clone RU1); Fab Fragment | Neut, FuncS | Human Fab |
| HPAB-S0146-YC-F(E) | Human Anti-Toxin Recombinant Antibody (clone LR); Fab Fragment | Neut, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0145-YC-S(P) | Human Anti-Toxin Recombinant Antibody (clone RU1); scFv Fragment | Neut, FuncS | Human scFv |
| HPAB-S0146-YC-S(P) | Human Anti-Toxin Recombinant Antibody (clone LR); scFv Fragment | Neut, FuncS | Human scFv |
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