Chicken Anti-USUV E DIII Recombinant Antibody (clone D2.2) (CAT#: VS-0323-CJ65)
This chicken anti-USUV E DIII antibody (clone D2.2) is a product that recognizes Usutu virus (USUV) envelope protein domain III.
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Custom Production
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ELISA
Figure 1 Binding of scFv to E protein DIII antigens in an ELISA.
The E protein DIII antigens of USUV A, B, I and WNV, were coated on high-binding ELISA plates. BSA in PBS was used as a negative control.
SPR
Figure 2 Responses and kinetic fits of VS-0323-CJ65 with E protein DIII from USUA and USUB.
VS-0323-CJ65 was immobilized via its His-tag and the binding to seven two-fold dilutions of the analyte (E protein DIII) was measured.
SPR
Figure 3 Responses and kinetic fits of VS-0323-CJ65 and E protein DIII from USUV and WNV.
WB
Figure 4 Western blot titrations of VS-0323-CJ65 using E protein DIII of USUV as antigens.
Immunoblots of decreasing amounts of USUV E protein DIII from groups A, B, and I E protein DIII were incubated with the VS-0323-CJ65 at a dilution of 1:2,000, followed by mouse anti-HA antibodies, and HRP-conjugated goat anti-mouse antibodies for visualization.
Neut
Figure 5 Virus ELISA binding assay on USUV Vienna‐2001.
A virus ELISA was performed with all five scFvs (VS-0323-CJ63, VS-0323-CJ64, VS-0323-CJ65, VS-0323-CJ66, A9) in two-fold dilution steps (2.5-20 μg/ml).
Specifications
- Immunogen
- Purified Usutu virus envelope protein domain III
- Host Species
- Chicken
- Type
- Chicken IgY
- Specificity
- Usutu virus (USUV) envelope protein domain III
- Species Reactivity
- USUV
- Clone
- D2.2
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Surface Plasmon Resonance, Neutralization
- Conjugate
- Unconjugated
- Related Disease
- Encephalitis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Surface Plasmon Resonance: 190 nM-2.5 μM
Western Blot: 1/500-1/2000
ELISA: 2.5 μg/mL-20 μg/mL
Target
- Alternative Names
- Usutu virus envelope protein domain III
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0323-CJ63 | Chicken Anti-USUV E DIII Recombinant Antibody (clone A8) | ELISA, WB, SPR, Neut | Chicken IgY |
| VS-0323-CJ64 | Chicken Anti-USUV E DIII Recombinant Antibody (clone A12) | ELISA, WB, SPR, Neut | Chicken IgY |
| VS-0323-CJ66 | Chicken Anti-USUV E DIII Recombinant Antibody (clone G1) | ELISA, WB, SPR, Neut | Chicken IgY |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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