Chicken Anti-USUV E DIII Recombinant Antibody (clone A8) (CAT#: VS-0323-CJ63)
This chicken anti-USUV E DIII antibody (clone A8) is a product that recognizes Usutu virus (USUV) envelope protein domain III.
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ELISA
Figure 1 Binding of scFv to E protein DIII antigens in an ELISA.
The E protein DIII antigens of USUV A, B, I and WNV, were coated on high-binding ELISA plates. BSA in PBS was used as a negative control.
SPR
Figure 2 Responses and kinetic fits of VS-0323-CJ63 and E protein DIII from USUA and USUB.
VS-0323-CJ63 was immobilized via its His-tag and the binding to seven two-fold dilutions of the analyte (E protein DIII) was measured.
SPR
Figure 3 Responses and kinetic fits of VS-0323-CJ63 and E protein DIII from USUV and WNV.
WB
Figure 4 Western blot titrations of VS-0323-CJ63 using E protein DIII of USUV as antigens.
Immunoblots of decreasing amounts of USUV E protein DIII from groups A, B, and I E protein DIII were incubated with the VS-0323-CJ63 at a dilution of 1:2,000, followed by mouse anti-HA antibodies, and HRP-conjugated goat anti-mouse antibodies for visualization.
Neut
Figure 5 Virus ELISA binding assay on USUV Vienna‐2001.
A virus ELISA was performed with all five scFvs (VS-0323-CJ63, VS-0323-CJ64, VS-0323-CJ65, VS-0323-CJ66, A9) in two-fold dilution steps (2.5-20 μg ml−1).
Specifications
- Immunogen
- Purified Usutu virus envelope protein domain III
- Host Species
- Chicken
- Type
- Chicken IgY
- Specificity
- Usutu virus (USUV) envelope protein domain III
- Species Reactivity
- USUV
- Clone
- A8
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Surface Plasmon Resonance, Neutralization
- Conjugate
- Unconjugated
- Related Disease
- Encephalitis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Surface Plasmon Resonance: 60 nM-3300 nM
Western Blot: 1/00-1/2000
ELISA: 2.5 μg/mL-20 μg/mL
Target
- Alternative Names
- Usutu virus envelope protein domain III
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0323-CJ64 | Chicken Anti-USUV E DIII Recombinant Antibody (clone A12) | ELISA, WB, SPR, Neut | Chicken IgY |
| VS-0323-CJ65 | Chicken Anti-USUV E DIII Recombinant Antibody (clone D2.2) | ELISA, WB, SPR, Neut | Chicken IgY |
| VS-0323-CJ66 | Chicken Anti-USUV E DIII Recombinant Antibody (clone G1) | ELISA, WB, SPR, Neut | Chicken IgY |
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