Human Anti-FLT4 scFv-Fc Chimera (VS-0425-FY54)
CAT#: VS-0425-FY54
The anti-FLT4 scFv-Fc fusion protein is engineered to target Fms-like tyrosine kinase 4 (FLT4), also known as VEGFR-3, a key regulator of lymphangiogenesis. This protein combines a single-chain variable fragment (scFv) for high-specificity binding to FLT4 with an Fc domain to enhance stability and extend its circulating half-life. By inhibiting FLT4 signaling, it aims to modulate lymphatic vessel development and function, potentially impacting conditions like lymphedema and tumor metastasis. The Fc region allows for extended systemic exposure, increasing potential therapeutic efficacy. Current investigations are evaluating its ability to precisely modulate lymphangiogenesis for therapeutic purposes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46519/1909_G1_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/99/if_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67906/153589_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67906/153589_A_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AN3-CA, BEWO, HEK93, HL-60, HUVEC TERT2, JURKAT, T-47d, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000037280-FLT4
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human FLT4
- Species Reactivity
- Human
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Fms Related Tyrosine Kinase 4; Tyrosine-Protein Kinase Receptor FLT4; Fms-Like Tyrosine Kinase 4; EC 2.7.10.1; VEGFR-3; VEGFR3; FLT-4
- Gene ID
- 2324
- UniProt ID
- P35916
- Long Name
- Fms Related Tyrosine Kinase 4
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Cellular Localization
- Cell membrane; Single-pass type I membrane protein; Cytoplasm; Nucleus
- Post Translation Modifications
- Autophosphorylated on tyrosine residues upon ligand binding.
Glycosylation at Asn33, Asn104, Asn166, Asn251, Asn299, Asn411, Asn515, Asn527, Asn594, Asn683, Asn690, and Asn758
Ubiquitination at Lys1253
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 13 sites, 5 N-linked glycans (5 sites)
- Protein Refseq
- XP_011532780.1; XP_011532786.1; XP_016864752.1; XP_016864755.1; XP_016864757.1
- Function
- Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development.
Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting.
Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling.
Modulates KDR signaling by forming heterodimers.
The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis.
Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites.
Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway.
Phosphorylates SHC1.
Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase.
Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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