Recombinant Mouse Anti-CDKN1B Antibody (clone 5H1-8C7-4C7)
CAT#: VS3-FY353
This product is a recombinant mouse antibody that recognizes human CDKN1B protein. The mAb was purified by affinity chromatography.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59086/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21888/48637_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111276-CDKN1B
❮
❯
IHC
Figure 1. Recombinant Mouse Anti-CDKN1B Antibody (clone 5H1-8C7-4C7) in IHC.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using p27 Kip 1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
IHC
Figure 2. Recombinant Mouse Anti-CDKN1B Antibody (clone 5H1-8C7-4C7) in IHC.
Immunohistochemical analysis of paraffin-embedded tonsil using p27 Kip 1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
❮
❯
Specifications
- Immunogen
- Synthesized peptide derived from human p27Kip1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CDKN1B
- Species Reactivity
- Human
- Clone
- 5H1-8C7-4C7
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- PBS pH 7.3, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/100-1/500
Target
- Alternative Names
- Cyclin Dependent Kinase Inhibitor 1B; Cyclin-Dependent Kinase Inhibitor 1B (P27, Kip1); P27KIP1; KIP1; Cyclin-Dependent Kinase Inhibitor P27; Cyclin-Dependent Kinase Inhibitor 1B; CDKN4; MEN1B; MEN4
- Gene ID
- 1027
- UniProt ID
- P46527
- Sequence Similarities
- Belongs to the CDI family.
- Cellular Localization
- Cytoplasm, Endosome, Nucleus
- Post Translation Modifications
- Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G0-G1 phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity).
- Protein Refseq
- NP_004055.1
- Function
- Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995).
Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY353. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "CDKN1B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1429CT | Recombinant Mouse anti-Human CDKN1B Monoclonal antibody (EML014) | ICC, IF, IHC-P, WB, sELISA | |
| ZG-0798F | Mouse Anti-CDKN1B Recombinant Antibody (clone DCS72) | WB, IP, IHC-P | Mouse IgG1 |
| VS3-QX219 | Mouse Anti-CDKN1B Recombinant Antibody (clone 5B4-E6-E11-A8) | WB | Mouse IgG1 |
| VS3-QX220 | Mouse Anti-CDKN1B Recombinant Antibody (clone 1373CT407.103.103) | WB, IHC | Mouse IgG1, κ |
| VS3-XY479 | Mouse Anti-CDKN1B Recombinant Antibody (clone 3D8) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0115MZ | Chicken Anti-CDKN1B Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0640 | Hi-Affi™ Rabbit Anti-CDKN1B Recombinant Antibody (clone DS640AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY359 | Mouse Anti-NHP CDKN1B Recombinant Antibody (clone SPM348) | IHC, ICC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY412 | Anti-CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1335 | Anti-Mouse CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1334 | Anti-Human CDKN1B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1336 | Anti-Canine CDKN1B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC202 | CytoStream™ Rabbit Anti-CDKN1B Recombinant Antibody (VS13-YC202) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
-CB2006C17L-4.jpg)
Application: WB, ELISA
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: FC, IHC-Fr, IP, ELISA, Block
Application: ELISA, IHC, FC, IP, IF, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
