Recombinant Mouse Anti-COL18A1 Detection Antibody (clone 5E3) (CAT#: VS3-WK1629)
This antibody is a mouse recombinant antibody against human COL18A1. The sandwich ELISA was established using matched antibody pair 12G3 and 5E3. The clone 5E3 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human COL18A1 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2a.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36104/if_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36104/if_selected.jpg
Figure 2 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong cytoplasmic and extracellular positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_8_4_selected.jpg
(Immunohistochemical staining of human liver shows strong cytoplasmic and extracellular positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_8_4_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_B_8_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_B_8_5.jpg
Figure 4 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_7_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_7_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_7_4.jpg
(Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_7_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+, EFO-21, NTERA-2, RPTEC TERT1, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182871-COL18A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182871-COL18A1
Specifications
- Immunogen
- Recombinant human COL18A1 protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human COL18A1
- Species Reactivity
- Human
- Clone
- 5E3
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Collagen Type XVIII Alpha 1 Chain; Collagen, Type XVIII, Alpha 1; Endostatin; Collagen Alpha-1(XVIII) Chain Isoform 1 Preproprotein; Collagen Alpha-1(XVIII) Chain; Multi-Functional Protein MFP
- Gene ID
- 80781
- UniProt ID
- P39060
- Long Name
- Collagen Type XVIII Alpha 1 Chain
- Sequence Similarities
- Belongs to the multiplexin collagen family.
- Cellular Localization
- Basement membrane, Extracellular matrix, Secreted
- Post Translation Modifications
- Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated.
Circulating endostatins are found as sialoglycoprotein and asialoglycoprotein structures.
- Protein Refseq
- NP_085059.2
- Function
- Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3275z | Mouse Anti-COL18A1 Recombinant Antibody (clone 38B11) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| VS3-WK1628 | Recombinant Mouse Anti-COL18A1 Capture Antibody (clone 12G3) | ELISA | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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