Recombinant Rabbit Anti-LIF Capture Antibody (clone R05-1S2)
CAT#: VS3-WK1746
This antibody is a rabbit recombinant antibody against human LIF. It was developed by immunization of rabbit with recombinant human LIF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R05-1S2 and R05-2T4. The clone R05-1S2 can be used as a capture antibody in ELISA analysis to identify human LIF.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25985/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic positivity in chief cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_8_4.jpg
Normal Tissue
Figure 7 Gallbladder
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_6_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128342-LIF
❮
❯
Specifications
- Immunogen
- Recombinant human LIF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LIF
- Species Reactivity
- Human
- Clone
- R05-1S2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- LIF, Interleukin 6 Family Cytokine
2
3
5
Differentiation Inhibitory Activity 2
3
Differentiation-Stimulating Factor 3
4
Cholinergic Differentiation Factor 2
3
Hepatocyte-Stimulating Factor III 2
3
Differentiation-Inducing Factor 2
3
Melanoma-Derived LPL Inhibitor 3
4
Human Interleukin In DA Cells 2
3
- Gene ID
- 3976
- UniProt ID
- P15018
- Long Name
- LIF, Interleukin 6 Family Cytokine
- Sequence Similarities
- Belongs to the LIF/OSM family.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_001244064.1
- Function
- LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
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Related Signaling Pathways
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "LIF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1815z | Mouse Anti-LIF Recombinant Antibody (clone 30C8) | ELISA, IHC, Neut | Mouse IgG2b |
| MOB-1091CT | Recombinant Mouse anti-Human LIF Monoclonal antibody (5G8.3) | WB | |
| VS3-WK1747 | Recombinant Rabbit Anti-LIF Detection Antibody (clone R05-2T4) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1691CQ | Recombinant Mouse Anti-LIF Antibody (CBL136) | IHC, Neut | IgG2 |
| NEUT-1692CQ | Recombinant Mouse Anti-LIF Antibody (CBL416) | IHC, ELISA(Cap), Neut | IgG2b |
| NEUT-1693CQ | Recombinant Mouse Anti-LIF Antibody (MM0454-6H31) | Neut, IHC, IHC-P | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4025 | Anti-LIF Immunohistochemistry Kit | IHC |
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