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    Recombinant Rabbit Anti-LIF Capture Antibody (clone R05-1S2) (CAT#: VS3-WK1746)

    This antibody is a rabbit recombinant antibody against human LIF. It was developed by immunization of rabbit with recombinant human LIF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R05-1S2 and R05-2T4. The clone R05-1S2 can be used as a capture antibody in ELISA analysis to identify human LIF.

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/if_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25985/if_selected.jpg
    Figure 3 IHC staining of human stomach
    Figure 3 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows cytoplasmic positivity in chief cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/ihc_selected.jpg
    Normal Tissue
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Glial cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_B_8_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_9_3.jpg
    Figure 6 Liver
    Figure 6 Liver
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_8_4.jpg
    Figure 7 Gallbladder
    Figure 7 Gallbladder
    (Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_6_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_8_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_4_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18844/43353_A_7_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Cell line enhanced.
    Figure 11 RNA cell line category: Cell line enhanced.
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128342-LIF

    Specifications

    • Immunogen
    • Recombinant human LIF protein
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human LIF
    • Species Reactivity
    • Human
    • Clone
    • R05-1S2
    • Applications
    • ELISA
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • 0.01M PBS, pH 7.2
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Target

    • Alternative Names
    • LIF, Interleukin 6 Family Cytokine
      2
      3
      5



      Differentiation Inhibitory Activity 2
      3


      Differentiation-Stimulating Factor 3
      4


      Cholinergic Differentiation Factor 2
      3


      Hepatocyte-Stimulating Factor III 2
      3


      Differentiation-Inducing Factor 2
      3


      Melanoma-Derived LPL Inhibitor 3
      4


      Human Interleukin In DA Cells 2
      3
    • Long Name
    • LIF, Interleukin 6 Family Cytokine
    • Sequence Similarities
    • Belongs to the LIF/OSM family.
    • Cellular Localization
    • Secreted
    • Function
    • LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

    Recommended Products

    Detection antibody

    Isotype Control

    Secondary Antibody

    Related Resources

    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "LIF"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibodies Neutralizing Antibodies IHC Kits & Antibodies
    CAT Product Name Application Type
    MOB-1815z Mouse Anti-LIF Recombinant Antibody (clone 30C8) ELISA, IHC, Neut Mouse IgG2b
    MOB-1091CT Recombinant Mouse anti-Human LIF Monoclonal antibody (5G8.3) WB
    VS3-WK1747 Recombinant Rabbit Anti-LIF Detection Antibody (clone R05-2T4) ELISA Rabbit IgG

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