Recombinant Rabbit Anti-STK3 Antibody (clone 3C3)

CAT#: VS4-CJ128

This rabbit anti-STK3 antibody (clone 3C3) is a product that recognizes human STK3. The immunogen of this antibody is a synthesized peptide derived from human STK3. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000).

Gene Expression
Figure 1 IF staining of human cell line U-2 OS Figure 2 IHC staining of human breast Figure 3 Cerebral cortex Figure 4 Colon Figure 5 Liver Figure 6 Kidney Figure 7 Testis Figure 8 Lymph node Figure 9 RNA cell line category: Cell line enhanced (A-431)

Specifications

  • Immunogen
  • A synthesized peptide derived from human STK3
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human STK3
  • Species Reactivity
  • Human
  • Clone
  • 3C3
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000).

Target

  • Alternative Names
  • KRS1; MST2
  • Long Name
  • Serine/threonine kinase 3
  • Sequence Similarities
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1.
    Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).
  • Function
  • Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity).
    Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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COA

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