Recombinant Mouse Anti-TRIM33 Antibody (VS4-CJ142)
CAT#: VS4-CJ142
This mouse anti-TRIM33 antibody is a product that recognizes human, dog, porcine, mouse, rat TRIM33. The immunogen of this antibody is recombinant fragment, corresponding to amino acids 712aa-853aa of human TRIM33 (C-terminus). The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4345/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4345/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4345/13245_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4345/13245_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4345/13245_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4345/13245_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4345/13245_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197323-TRIM33
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Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 712aa-853aa of human TRIM33 (C-terminus).
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human, Dog, Porcine, Mouse, Rat TRIM33
- Species Reactivity
- Human, Dog, Porcine, Mouse, Rat
- Applications
- ELISA, WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.
- Preservative
- 0.2% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Target
- Alternative Names
- ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA
- Gene ID
- 51592
- UniProt ID
- Q9UPN9
- Long Name
- Tripartite motif containing 33
- Sequence Similarities
- Belongs to the TRIM/RBCC family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylated with SUMO1.
- Protein Refseq
- NP_056990.3; NP_148980.2
- Function
- Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity).
Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
See other products for "TRIM33"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2541z | Mouse Anti-TRIM33 Recombinant Antibody (clone 38A9) | ELISA, WB | Mouse IgG2a |
| MOB-2340MZ | Recombinant Mouse Anti-Human TRIM33 Antibody (clone 16G9) | IHC-P, WB | Mouse antibody |
| VS3-QX1167 | Mouse Anti-TRIM33 Recombinant Antibody (clone 3H7-C8-B9) | WB | Mouse IgG2a |
| VS3-CJ88 | Mouse Anti-TRIM33 Recombinant Antibody (VS3-CJ88) | WB, ELISA | Mouse IgG |
| VS7-0425-WR1051 | Mouse Anti-TRIM33 Recombinant Antibody (VS7-0425-WR1051) | WB, IF, ICC | Mouse IgG |
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