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WNV-DIII
Anti-WNV-DIII Recombinant Antibody Products
- Recombinant Anti-WNV-DIII Antibody (MOB-1051)
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- Derivation: Mouse
- Species Reactivity: WNV
- Type: IgG
- Application: ELISA, WB, FuncS
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- Recombinant Human Anti-WNV-DIII Antibody (MHH-1051)
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- Derivation: Human
- Species Reactivity: WNV
- Type: IgG
- Application: Neut, WB, FuncS
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- Recombinant Human Anti-WNV-DIII Antibody Fab Fragment (MHH-1051-F(E))
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- Derivation: Human
- Species Reactivity: WNV
- Type: Fab
- Application: RIA, IF, FuncS
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- Recombinant Human Anti-WNV-DIII Antibody scFv Fragment (MHH-1051-S(P))
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- Derivation: Human
- Species Reactivity: WNV
- Type: scFv
- Application: WB, IF, FuncS
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- Recombinant Anti-WNV-DIII Antibody Fab Fragment (MOB-1051-F(E))
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- Derivation: Mouse
- Species Reactivity: WNV
- Type: Fab
- Application: FC, IP, FuncS
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- Recombinant Anti-WNV-DIII Antibody scFv Fragment (MOB-1051-S(P))
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- Derivation: Mouse
- Species Reactivity: WNV
- Type: scFv
- Application: IF, FC, Biosensors, FuncS
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For Research Use Only. Not For Clinical Use.
The involvement of domain III of West Nile virus (WNV-DIII) envelope protein in binding to host cell surface was investigated. WNV-DIII was first expressed as a recombinant protein and purified after a solubilization and refolding procedure. The refolded WNV-DIII protein displays a content of betasheets consistent with known homologous structures of other flavivirus envelope DIII, shown by using circular dichroism analysis. Purified recombinant WNV-DIII protein was able to inhibit WNV entry into Vero cells and C6/36 mosquito cells. Recombinant WNV-DIII only partially blocked the entry of dengue-2 (Den 2) virus into Vero cells. However, entry of Den 2 virus into C6/36 was blocked effectively by recombinant WNV-DIII. Murine polyclonal serum produced against recombinant WNV-DIII protein inhibited infection with WNV and to a much lesser extent with Den 2 virus, as demonstrated by plaque neutralization assays.