Hi-Affi™ Rabbit Anti-ARFGAP1 Recombinant Antibody (clone DS232AB)
CAT#: MOR-0232
Recombinant Rabbit anti-ARFGAP1 monoclonal antibody for WB, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, cytosol, the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51019/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cervix, uterine
Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/148206_B_8_5.jpg
Normal Tissue
Figure 5 Cervix
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/148206_B_8_3.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101199-ARFGAP1
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Specifications
- Immunogen
- Recombinant fragment within Human ARFGAP1 aa 300 to the C-terminus. The exact sequence is proprietary.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS232AB
- Applications
- WB, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ARFGAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK32 | Mouse Anti-ARFGAP1 Recombinant Antibody (clone E2-C4) | WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY33 | Mouse Anti-NHP ARFGAP1 Recombinant Antibody (clone OTI1F6) | WB, IHC, IF, FC | Mouse IgG2b |
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