Recombinant Mouse Anti-AdV Fiber Antibody (4D2) (CAT#: MRO-378CQ)
This antibody is a mouse monoclonal antibody that is capable of binding to AdV Fiber.
Specific Inquiry
FuncS
Figure 1 Fiber trimers were analyzed by SDS-PAGE and NDS-PAGE and immunoblotting with 4D2.5 antibody as boiled.
Recombinant fibers, ability to trimerize.
Magnusson, M. K., Hong, S. S., Boulanger, P., & Lindholm, L. (2001). Genetic retargeting of adenovirus: novel strategy employing “deknobbing” of the fiber. Journal of virology, 75(16), 7280-7289.
ELISA
Figure 2 Fiber-binding properties in vitro tested by ELISA.
Wells were coated with anti-fiber tail monoclonal antibody 4D2.5, WT or R7-RGD fiber was added.
Magnusson, M. K., Hong, S. S., Boulanger, P., & Lindholm, L. (2001). Genetic retargeting of adenovirus: novel strategy employing “deknobbing” of the fiber. Journal of virology, 75(16), 7280-7289.
ELISA
Figure 3 ELISA-based binding assay of zipper-tagged 566FF fibers with zipper-scFv G28-5.
The formation of stable complexes was demonstrated by detection of the scFv-bound 566FF fiber proteins with an anti-fiber 4D2 monoclonal antibody.
Glasgow, J. N., Mikheeva, G., Krasnykh, V., & Curiel, D. T. (2009). A strategy for adenovirus vector targeting with a secreted single chain antibody. PloS one, 4(12).
WB
Figure 4 Trimerization profiles of zipper-modified 566FF fiber proteins.
Western blot analysis of lysates of 293T cells transfected with expression vectors expressing zipper-modified 566FF fiber proteins.
Glasgow, J. N., Mikheeva, G., Krasnykh, V., & Curiel, D. T. (2009). A strategy for adenovirus vector targeting with a secreted single chain antibody. PloS one, 4(12).
IF
Figure 5 Indirect immunofluorescence of recombinant fiber mutants. Each recombinant was probed with the 4D2, 2A6, and RaF2Nat antibodies.
Two examples of fiber mutants that form trimers are shown. An internal deletion mutant, 2F-dlSph, represents a typical immunofluorescence pattern of trimeric
fiber in the nuclei of recombinant virus-infected cells.
Hong, J. S., & Engler, J. A. (1996). Domains required for assembly of adenovirus type 2 fiber trimers. Journal of virology, 70(10), 7071-7078.
Specifications
- Immunogen
- Adenovirus type 2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse antibody
- Specificity
- Adenovirus Fiber monomer and trimer
- Species Reactivity
- Adenovirus
- Clone
- 4D2
- Applications
- WB, ELISA, FuncS, IF
Product Property
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The AdV Fiber antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Immunofluorescence.
Target
- Alternative Names
- Adenovirus; Adeno; AdV; Fiber protein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-379CQ | Recombinant Mouse Anti-AdV Fiber Antibody (2A6) | WB, IF | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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