Recombinant monoclonal antibody specifically binds to Burkholderia pseudomallei Exotoxin, expressed in Chinese Hamster Ovary cells(CHO).
Figure 1 Western blot of B. pseudomallei antigens with thescFv antibody.
1: P. aeruginosa exotoxin A (negative control); 2:Purified B. pseudomallei exotoxin from 6E6A8F3B immobilizedcolumn; 3: Purified B. pseudomallei exotoxin from scFvimmobilized column; 4: Partially purified B. pseudomallei exotoxinfrom Sephadex G-100 chromatography; 5: Partially purified B.pseudomallei hemolysin from Sephadex G-100 chromatography;6: B. pseudomallei crude extract; M: Broad range protein marker.
Purification of A Burkholderia pseudomallei Antigen Via AntibodyMediated Affinity Chromatography
Figure 2 Western analysis of B. pseudomallei antigens blottedwith the 6E6A8F3B monoclonal antibody.
1: Purified B.pseudomallei exotoxin from scFv-immobilized column; 2: Puri-fied B. pseudomallei exotoxin from 6E6A8F3B immobilized col-umn; 3: Partially purified B. pseudomallei exotoxin from SephadexG-100 chromatography; 4: Partially purified B. pseudomalleihemolysin from Sephadex G-100 chromatography; 5: B.pseudomallei crude extract; 6: P. aeruginosa exotoxin A (negativecontrol); M: Broad range protein marker.
Purification of A Burkholderia pseudomallei Antigen Via AntibodyMediated Affinity Chromatography
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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