Recombinant Chimpanzee Anti-DENV-4 Antibody (mAb 5H2) (CAT#: PABL-448)
Recombinant Chimpanzee Antibody (mAb 5H2) is capable of binding to DENV-4 , expressed in Chinese Hamster Ovary cells (CHO). This antibody potently neutralizes DENV-4 by binding to domain I of E.
Specific Inquiry
ELISA
Figure 1 ELISA binding of DENV4-specific and cross-reactive monoclonal antibodies on rDENV4/3 panel.
ELISA binding curves of DENV3, DENV4, DENV4/3 M12, DENV4/3 M14 and DENV4/3 M16 by DENV4-specific mAbs 5H2.
Widman, D. G., Young, E., Nivarthi, U., Swanstrom, J. A., Royal, S. R., Yount, B. L., ... & De Silva, A. M. (2017). Transplantation of a quaternary structure neutralizing antibody epitope from dengue virus serotype 3 into serotype 4. Scientific reports, 7(1), 1-10.
Neut
Figure 2 Neutralization of DENV4-specific and cross-reactive monoclonal antibodies on rDENV4/3 panel.
U937-DC-SIGN Neutralization assays with DENV4-specific mAbs 5H2.
Widman, D. G., Young, E., Nivarthi, U., Swanstrom, J. A., Royal, S. R., Yount, B. L., ... & De Silva, A. M. (2017). Transplantation of a quaternary structure neutralizing antibody epitope from dengue virus serotype 3 into serotype 4. Scientific reports, 7(1), 1-10.
Neut
Figure 3 In vitro neutralization assay of IgG 5H2 ΔD against DENV-4 parental viruses and antigenic variants.
The multiplicity of infection was 0.1.
Lai, C. J., Goncalvez, A. P., Men, R., Wernly, C., Donau, O., Engle, R. E., & Purcell, R. H. (2007). Epitope determinants of a chimpanzee dengue virus type 4 (DENV-4)-neutralizing antibody and protection against DENV-4 challenge in mice and rhesus monkeys by passively transferred humanized antibody. Journal of virology, 81(23), 12766-12774.
Specifications
- Immunogen
- Dengue virus 4
- Host Species
- Chimpanzee
- Derivation
- Chimpanzee
- Type
- IgG
- Specificity
- Tested positive against native DENV-4
- Species Reactivity
- DENV
- Clone
- mAb 5H2
- Applications
- ELISA, Neut
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay and Neutralization.
Target
- Alternative Names
- Dengue virus; DENV; Flaviviridae; dengue virus 4; DENV 4; Flaviviridae
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-445 | Recombinant Chimpanzee Anti-DENV-4 Antibody Fab Fragment (mAb 5H2) | WB, ELISA, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-445 | Recombinant Chimpanzee Anti-DENV-4 Antibody scFv Fragment (mAb 5H2) | WB, ELISA, FuncS | scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-490CQ | Recombinant Mouse Anti-DENV-4 Antibody (2Q1901) | IF | Mouse antibody |
| MRO-491CQ | Recombinant Mouse Anti-DENV-4 Antibody (ICL4) | IF | Mouse antibody |
| MRO-492CQ | Recombinant Mouse Anti-DENV-4 Antibody (M8051124) | IF | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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