Recombinant Mouse Anti-E. coli ptsH Antibody (JEL42) (CAT#: PABW-036)

Figure 1 Monoclonal antibody titers. HPr was attached to microtiter plates.

Antibodies were serially diluted into the microtiter plates and incubated, and then the unbound antibody was washed off. Bound antibody was determined by using ¹²⁵I-labeled sheep anti-mouse IgG antibody. The monoclonal antibodies were Jel42 from culture fluid (*), Jel44 from culture fluid (A), and Jel42 purified
from ascites fluid (A).

Waygood, E. B., Reiche, B., Hengstenberg, W., & Lee, J. S. (1987). Characterization of mutant histidine-containing proteins of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium. Journal of bacteriology, 169(6), 2810-2818.

Figure 2 HPr levels in S. typhimurium strains. Competition SPRIA assays were carried out.

The results presented are the inhibition of antibody binding to the HPr bound to microtiter plates as a function of the amount of competing protein added to the
wells. The antibody preparation used in this experiment was a 1:4 dilution of the Jel42 from culture fluid. Samples: HPr (○); extracts of S. typhimurium SB3507 grown on nutrient broth (Δ), nutrient broth with glucose (A), or nutrient broth with fructose -- -, 0); extracts of S. typhimurium SB2226 grown on nutrient broth (O) or nutrient broth with fructose (O).

Waygood, E. B., Reiche, B., Hengstenberg, W., & Lee, J. S. (1987). Characterization of mutant histidine-containing proteins of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium. Journal of bacteriology, 169(6), 2810-2818.

Figure 3 Comparison of the antigenicity of pure HPr from S.typhimurium SB3093 with wild-type HPr.

Competition SPRIA assays were carried out that the antibody preparation for Jel42 was from ascites fluid (1:400). Wild-type HPr from E. coli (solid symbols) and HPr from S. typhimurium SB3093 (open symbols) were tested with Jel42 (triangles) and Jel44 (circles).

Waygood, E. B., Reiche, B., Hengstenberg, W., & Lee, J. S. (1987). Characterization of mutant histidine-containing proteins of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium. Journal of bacteriology, 169(6), 2810-2818.