Does this antibody neutralize TNF alpha?

Yes, this Human Anti-TNF scFv Fragment binds and neutralizes the transmembrane form of TNF alpha. It is potent against human, rhesus macaque, cynomolgus monkey, and canine TNF alpha.

How pure is this scFv fragment?

The product has a purity of greater than 95% as determined by SDS-PAGE. It is a recombinant protein suitable for ELISA, Flow Cytometry, and Neutralization assays.

Human Anti-TNF Recombinant Antibody; scFv Fragment (TAB-115CQ-S(P))

CAT#: TAB-115CQ-S(P)

This product is a recombinant human antibody scFv fragment that binds and neutralizes the transmembrane form of TNF alpha. The antibody was highly potent in neutralizing rhesus macaque, cynomolgus monkey and canine TNF alpha proteins.

Gene Expression

Normal Tissue Figure 1 Tonsil

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF

❮

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Specifications

Immunogen
Recombinant human TNF alpha

Host Species
Human

Derivation
Humanized

Type
Human scFv

Specificity
Human TNF

Species Reactivity
Human, Rhesus macaque, Cynomolgus, Canine

Applications
ELISA, FC, Neut

Related Disease
Inflammatory diseases

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNLG1F; Tumor necrosis factor

Gene ID
7124

UniProt ID
P01375

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Breast cancer biomarkers at key points during disease progression

Potent Neutralizer

We used this human scFv fragment to neutralize TNF alpha in our bioassays. It was highly potent, even against rhesus and canine TNF alpha. The cross-species reactivity is a huge benefit for our comparative immunology research. The recombinant format is very reliable.

High Purity scFv

The purity of this scFv product is excellent (>95%). We used it in Flow Cytometry to bind membrane-bound TNF. It gave distinct shifts and low background. It is a versatile reagent for studying inflammatory cytokines and their regulation in different animal models.

Q&As

Does this antibody neutralize TNF alpha?

A: Yes, this Human Anti-TNF scFv Fragment binds and neutralizes the transmembrane form of TNF alpha. It is potent against human, rhesus macaque, cynomolgus monkey, and canine TNF alpha.
How pure is this scFv fragment?

A: The product has a purity of greater than 95% as determined by SDS-PAGE. It is a recombinant protein suitable for ELISA, Flow Cytometry, and Neutralization assays.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Asthma

Inflammatory Bowel Diseases

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-115CQ-S(P).pdf

MSDS

MSDS - TAB-115CQ-S(P).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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