Anti-Yersinia V-antigen Recombinant Antibody (17A5.1) (MRO-147MZ) (CAT#: MRO-147MZ)

Figure 1 Immunoblots prepared with absorbed rabbit polyclonal anti-native V antigen (A), mouse monoclonal anti-V antigen 15A4.8 (B), and mouse monoclonal anti-V antigen 17A5.1 (C) against extracts of E. coli BL21(DE3)/pBluescript SK⁺ (lanes 1), E. coli BL21(DE3)/ pBVP515D (lanes 2), E. coli BL21(DE3)/pBVP514D (lanes 3), E. coli BL21(DE3)/pBVP53D (lanes 4), E. coli BL21(DE3)/pBVP513D (lanes 5), E. coli BL21(DE3)/pBVP5 (lanes 6), Lcr⁺ Y pestis KIM (lanes 7), and Lcr-Y pestis KIM (lanes 8). Numbers on the right indicate molecular masses in kilodaltons.

Passive immunity to Yersiniae mediated by anti-recombinant V-antigen and Protein A-V antigen fusion peptide

Figure 2 Immunoblots prepared with polyclonal anti-native V antigen (A) or mouse monoclonal anti-V antigen 17A5.1 (B) directed against truncated protein A (LANES 1),PAV (LANES 2),PAV partially hydrolyzed by formic acid and then passed through the IgG-Sepharose 6FF column (lane 4), whole Ca²⁺-starved Lcr⁺ cells of Y.pestis KIM (LANE 6).

Passive immunity to Yersiniae mediated by anti-recombinant V-antigen and Protein A-V antigen fusion peptide