Recombinant Human Anti-Siglec-15 Antibody (CAT#: MHH-905)

Figure 1 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in SDS-PAGE

SDS-PAGE analysis of MHH-905 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.

Figure 2 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in WB

Western blot analysis of MHH-905 was performed by loading Human Siglec-15 / CD33L3 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MHH-905 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)

Figure 3 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in DB

Dot Blot analysis of MHH-905 was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag).

MHH-905 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 4 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in ELISA

ELISA analysis of MHH-905 was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag). Then blocked with BSA, and incubated with MHH-905. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.