Recombinant Human Anti-Siglec-15 Antibody (CAT#: MHH-905)
Recombinant Human Antibody has specificity for an antigen Siglec-15, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Siglec-15 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Siglec-15 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
SDS-PAGE
Figure 1 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in SDS-PAGE
SDS-PAGE analysis of MHH-905 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
WB
Figure 2 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in WB
Western blot analysis of MHH-905 was performed by loading Human Siglec-15 / CD33L3 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MHH-905 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)
DB
Figure 3 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in DB
Dot Blot analysis of MHH-905 was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag).
MHH-905 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 4 Human Anti-SIGLEC15 Recombinant Antibody (MHH-905) in ELISA
ELISA analysis of MHH-905 was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag). Then blocked with BSA, and incubated with MHH-905. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Applications
- Formats of immunological tests: Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional Study
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Siglec-15
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Siglec-15"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-905 | Recombinant Anti-Siglec-15 Antibody | WB, IF, FuncS | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-905-F(E) | Recombinant Anti-Siglec-15 Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| MHH-905-F(E) | Recombinant Human Anti-Siglec-15 Antibody Fab Fragment | IF, FC, Biosensors, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-905-S(P) | Recombinant Anti-Siglec-15 Antibody scFv Fragment | WB, IP, FuncS | scFv |
| MHH-905-S(P) | Recombinant Human Anti-Siglec-15 Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for MHH-905. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.