Mouse Anti-ABCB4 Recombinant Antibody (VS3-FY2617)
CAT#: VS3-FY2617
The anti-ABCB4 antibody is a mouse recombinant monoclonal antibody that with high affinity for ABCB4 protein, a calculated weight of 141.5 kDa. It exhibits cross-reactivity with human, mouse and rat. This monoclonal antibody is potential for conducting research on Cholestasis, Progressive Familial Intrahepatic, 3 and Gallbladder Disease 1. The antibody can be utilized for detection of ABCB4 by Western Blot.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49395/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows strong positivity in bile canaliculi.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49395/124384_A_7_4_selected.jpg
Normal Tissue
Figure 3 High expression in liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53288/166934_A_8_4.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (SH-SY5Y, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005471-ABCB4
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- ATP binding cassette subfamily B member 4 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 141.5 kDa.
- Related Disease
- Cholestasis, Progressive Familial Intrahepatic, 3 and Gallbladder Disease 1
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Target
- Alternative Names
- ATP Binding Cassette Subfamily B Member 4; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 4; ATP-Binding Cassette Sub-Family B Member 4; Multidrug Resistance Protein 3; P-Glycoprotein 3; EC 3.6.3.44; MDR3; PGY3; P-Glycoprotein-3/Multiple Drug Resistance-3; Phosphatidylcholine Translocator ABCB4
- Gene ID
- 5244
- UniProt ID
- P21439
- Sequence Similarities
- Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
- Cellular Localization
- Cell membrane; Multi-pass membrane protein
Apical cell membrane; Multi-pass membrane protein
Membrane raft
Cytoplasm
- Post Translation Modifications
- Phosphorylated.
Phosphorylation on Thr-34 is required for PC efflux activity.
Phosphorylation occurs on serine and threonine residues in a protein kinase A- or C-dependent manner.
May be phosphorylated on Thr-44 and Ser-49.
Glycosylated.
Glycosylation at Asn91 and Asn97
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 2 sites
- Protein Refseq
- XP_011514610.4; XP_011514611.4; XP_047276431.1; XP_047276432.1; XP_047276433.1
- Function
- Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes.
Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ABCB4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0520MZ | Mouse Anti-ABCB4 Recombinant Antibody (clone Q4JJ-37) | FC, ICC, IHC-Fr, WB | Mouse IgG2b |
| VS3-QX3 | Mouse Anti-ABCB4 Recombinant Antibody (clone 1808CT346.21.23) | WB | Mouse IgG1, κ |
| VS-0824-YJ43 | Human Anti-ABCB4 Recombinant Antibody (VS-0824-YJ43) | ELISA | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC371 | Recombinant Anti-ABCB4 Vesicular Antibody, EV Displayed (VS-0425-YC371) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY19 | Anti-ABCB4 Immunohistochemistry Kit | IHC |
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