Mouse Anti-ACTG1 Recombinant Antibody (VS3-CJ1066) (CAT#: VS3-CJ1066)

• Immunogen
• KLH-conjugated synthetic peptide encompassing a sequence within human Actin pan

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human ACTG1

• Species Reactivity
• Human

• Applications
• WB, IHC

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.2% BSA, PBS.

• Preservative
• 0.01% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:1000), Immunohistochemistry (1:100-1:300).

• Alternative Names
• ACT; ACTG; DFNA20; DFNA26; HEL-176

• Gene ID
• 71

• UniProt ID
• P63261

• Sequence Similarities
• Belongs to the actin family.

• Cellular Localization
• Cytoplasm, Cytoskeleton

• Post Translation Modifications
• Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.
  Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
  Actin, cytoplasmic 2, N-terminally processed
  N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins (PubMed:29581253).
  In contrast, filament nucleation by the Arp2/3 complex is not affected (PubMed:29581253).
  Methylated at His-73 by SETD3.
  (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515).
  The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

• Protein Refseq
• NP_001186883.1; NP_001605.1

• Function
• Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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