Human Anti-BTN3A1 Recombinant Antibody (clone CTX-2026) (CAT#: VS-0524-YC3)
Clone CTX-2026 is a novel human antibody targeting BTN3A1. CTX-2026 partially share an overlapping epitope with anti-BTN3A1 mAb 20.1. Clone CTX-2026 is effective at restoring pMHC:TCR. CTX-2026 also blocked human BTN3A1-mediated suppression of mouse αβ T-cells. CTX-2026 is sufficient to elicit coordinated αβ and γδ T-cell anti-tumor responses.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/if_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in a granular pattern in renal tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in a granular pattern in renal tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_B_7_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_3.jpg
Figure 5 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_5.jpg
Figure 7 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000026950-BTN3A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000026950-BTN3A1
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human Butyrophilin Subfamily 3 Member A1 (BTN3A1)
- Species Reactivity
- Human
- Clone
- CTX-2026
- Applications
- ELISA, Block, FuncS
- Related Disease
- Tumors
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- CTX-2026 (1 μg/mL) enhanced antigen-specific killing of NY-ESO-1-transduced, HLA-A2⁺BTN3A1⁺ OVCAR3 cells (NY-OVCAR3).
CTX-2026 (1 μg/mL) heightened cytotoxic elimination of BTN3A1⁺ primary cancer cells from 3 HGSOCs by autologous tumor-infiltrating lymphocytes.
CTX-2026 (1 μg/mL) re-directed the cytotoxic activity of Vγ9Vδ2 T-cells from multiple donors against BTN3A1⁺ OVCAR3 cells, or primary HGSOC cells.
CTX-2026 (5mg/kg; i.p.) delayed malignant progression in donors by blocking BTN3A1.
CTX-2026 (5mg/kg; i.p.) blocked human BTN3A1-mediated suppression of mouse αβ T-cells.
Target
- Alternative Names
- Butyrophilin Subfamily 3 Member A1; BTF5; DJ45P21.3 (Butyrophilin, Subfamily 3, Member A1); Butyrophilin, Subfamily 3, Member A1; CD277 Antigen; BTN3.1; BT3.1; CD277;
- Gene ID
- 11119
- UniProt ID
- O00481
- Long Name
- Butyrophilin Subfamily 3 Member A1
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- N-glycosylation
Glycosylation at Asn115
- Protein Refseq
- XP_005248890.1; XP_005248891.1; XP_047274076.1; XP_047274077.1; XP_047274078.1
- Function
- Regulates the proliferation of activated T-cells.
Regulates the release of cytokines and IFNG by activated T-cells.
Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites.
Recommended Products
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-127-F(E) | Recombinant Anti-human BTN3A1 Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| MHH-127-F(E) | Recombinant Human Anti-human BTN3A1 Antibody Fab Fragment | IP, IF, FuncS | Fab |
| PFBL-411 | Mouse Anti-BTN3A1 Recombinant Antibody (clone 20.1); Fab Fragment | FC, Block | Mouse Fab |
| PFBL-412 | Mouse Anti-BTN3A1 Recombinant Antibody (clone 103.2); Fab Fragment | FC, Block | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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