Human Anti-BTN3A1 Recombinant Antibody (clone CTX-2026) (CAT#: VS-0524-YC3)
Clone CTX-2026 is a novel human antibody targeting BTN3A1. CTX-2026 partially share an overlapping epitope with anti-BTN3A1 mAb 20.1. Clone CTX-2026 is effective at restoring pMHC:TCR. CTX-2026 also blocked human BTN3A1-mediated suppression of mouse αβ T-cells. CTX-2026 is sufficient to elicit coordinated αβ and γδ T-cell anti-tumor responses.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/if_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in a granular pattern in renal tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in a granular pattern in renal tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_B_7_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_3.jpg
Figure 5 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_5.jpg
Figure 7 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12565/40150_A_9_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000026950-BTN3A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000026950-BTN3A1
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human Butyrophilin Subfamily 3 Member A1 (BTN3A1)
- Species Reactivity
- Human
- Clone
- CTX-2026
- Applications
- ELISA, Block, FuncS
- Related Disease
- Tumors
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- CTX-2026 (1 μg/mL) enhanced antigen-specific killing of NY-ESO-1-transduced, HLA-A2⁺BTN3A1⁺ OVCAR3 cells (NY-OVCAR3).
CTX-2026 (1 μg/mL) heightened cytotoxic elimination of BTN3A1⁺ primary cancer cells from 3 HGSOCs by autologous tumor-infiltrating lymphocytes.
CTX-2026 (1 μg/mL) re-directed the cytotoxic activity of Vγ9Vδ2 T-cells from multiple donors against BTN3A1⁺ OVCAR3 cells, or primary HGSOC cells.
CTX-2026 (5mg/kg; i.p.) delayed malignant progression in donors by blocking BTN3A1.
CTX-2026 (5mg/kg; i.p.) blocked human BTN3A1-mediated suppression of mouse αβ T-cells.
Target
- Alternative Names
- Butyrophilin Subfamily 3 Member A1; BTF5; DJ45P21.3 (Butyrophilin, Subfamily 3, Member A1); Butyrophilin, Subfamily 3, Member A1; CD277 Antigen; BTN3.1; BT3.1; CD277;
- Gene ID
- 11119
- UniProt ID
- O00481
- Long Name
- Butyrophilin Subfamily 3 Member A1
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- N-glycosylation
Glycosylation at Asn115
- Protein Refseq
- XP_005248890.1; XP_005248891.1; XP_047274076.1; XP_047274077.1; XP_047274078.1
- Function
- Regulates the proliferation of activated T-cells.
Regulates the release of cytokines and IFNG by activated T-cells.
Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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