Rabbit Anti-CAPN1 Recombinant Antibody (clone R08-2K8)
CAT#: VS3-XY3287
The rabbit recombinant antibody specifically binds to CAPN1 (Calpain 1). It recognizes human CAPN1 and it also has cross-reactivity against mouse and rat CAPN1. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/if_selected.jpg
Normal Tissue
Figure 2 High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_4_8_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5992/16388_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000014216-CAPN1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CAPN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-2K8
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Spastic paraplegia 76, autosomal recessive (SPG76)
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- CANP; muCL; CANP1; SPG76; CANPL1; muCANP
- Gene ID
- 823
- UniProt ID
- P07384
- Long Name
- Calpain 1
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
- Protein Refseq
- XP_006718761.1
- Function
- Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'.
Cleaves and activates caspase-7.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CAPN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0460 | Hi-Affi™ Rabbit Anti-CAPN1 Recombinant Antibody (clone DS460AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0215-CN | Mouse Anti-CAPN1 Recombinant Antibody (clone 2A1) | IHC, FC | Mouse IgG1 |
| MRO-1712-CN | Rabbit Anti-CAPN1 Polyclonal Antibody (MRO-1712-CN) | WB, IF, IHC | Rabbit IgG |
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| VS3-QX150 | Mouse Anti-CAPN1 Recombinant Antibody (clone 1376CT809.24.85) | WB, IHC, FC | Mouse IgG1 |
| VS7-HM290 | Mouse Anti-CAPN1 Recombinant Antibody (clone 1E6G9) | FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0216-CN | Mouse Anti-CAPN1 Recombinant Antibody (clone 2A2) | IF, IHC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1126 | AbPlus™ Anti-CAPN1 Magnetic Beads (VS-0724-YC1126) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC119 | CytoStream™ Mouse Anti-CAPN1 Recombinant Antibody (VS13-YC119) | ICC, IF, IHC-P, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY971 | Anti-CAPN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY972 | Anti-Mouse CAPN1 Immunohistochemistry Kit | IHC |
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