Rabbit Anti-CASP6 Recombinant Antibody (clone R04-5F9) (CAT#: VS3-FY1594)

• Immunogen
• Recombinant human Caspase-6 protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat CASP6

• Species Reactivity
• Human, Rat

• Clone
• R04-5F9

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 11,33 kDa.

• Related Disease
• Huntington Disease and Colorectal Cancer

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Caspase 6; Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; EC 3.4.22.59; MCH2; Apoptotic Protease MCH-2

• Gene ID
• 839

• UniProt ID
• P55212

• Sequence Similarities
• Belongs to the peptidase C14A family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylated by NUAK1; phosphorylation inhibits self-activation (PubMed:15273717, PubMed:22483120)., Phosphorylation at Ser-257 by AMP-activated protein kinase (PRKAA1 or PRKAA2) inhibits autocleavage, preventing caspase activation, thereby preventing hepatocyte apoptosis (PubMed:32029622)., Palmitoylation by ZDHHC17 blocks dimerization and subsequent activation, leading to inhibit the cysteine protease activity., Can be cleaved and activated by different caspases, depending on the context (PubMed:19133298, PubMed:28864531)., Cleaved and activated by caspase-8 (CASP8) and subsequently by caspase-3 (CASP3) (PubMed:9463409)., Can also undergo autoactivation by mediating autocleavage at Asp-179 and Asp-193, while it is not able to cleave its N-terminal disordered prodomain (PubMed:19133298, PubMed:28864531)., Cleaved and activated by CASP1, possibly in the context of inflammation (PubMed:16123779).

• Protein Refseq
• NP_001217.2; NP_116787.1

• Function
• Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis.
  Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity).
  Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death.
  Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity).
  Regulates B-cell programs both during early development and after antigen stimulation (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody