Rabbit Anti-CASP6 Recombinant Antibody (clone R04-5F9)
CAT#: VS3-FY1594
The R04-5F9 recombinant monoclonal antibody detects human CASP6. It cross-reacts with rat. This caspase 6 antibody was used in western blot analysis of caspase6 in Jurkat, C6 lysates. Western blot demonstrated that R04-5F9 recognized a target protein with a molecular weight of 33 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11337/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3775/if_selected.jpg
Normal Tissue
Figure 3 Small intestine
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11337/29069_A_5_2_val_selected.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_7_5.jpg
Normal Tissue
Figure 5 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_4_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_7_8.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_9_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138794-CASP6
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WB
Figure 1 Anti-CASP6 Antibody (VS3-FY1594) in WB.
The Caspase6 expression in Jurkat, C6 lysates were detected using the anti-Caspase 6 (CASP6) by western blot assay.
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Specifications
- Immunogen
- Recombinant human Caspase-6 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CASP6
- Species Reactivity
- Human, Rat
- Clone
- R04-5F9
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 11,33 kDa.
- Related Disease
- Huntington Disease and Colorectal Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Caspase 6; Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; EC 3.4.22.59; MCH2; Apoptotic Protease MCH-2
- Gene ID
- 839
- UniProt ID
- P55212
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated by NUAK1; phosphorylation inhibits self-activation (PubMed:15273717, PubMed:22483120)., Phosphorylation at Ser-257 by AMP-activated protein kinase (PRKAA1 or PRKAA2) inhibits autocleavage, preventing caspase activation, thereby preventing hepatocyte apoptosis (PubMed:32029622)., Palmitoylation by ZDHHC17 blocks dimerization and subsequent activation, leading to inhibit the cysteine protease activity., Can be cleaved and activated by different caspases, depending on the context (PubMed:19133298, PubMed:28864531)., Cleaved and activated by caspase-8 (CASP8) and subsequently by caspase-3 (CASP3) (PubMed:9463409)., Can also undergo autoactivation by mediating autocleavage at Asp-179 and Asp-193, while it is not able to cleave its N-terminal disordered prodomain (PubMed:19133298, PubMed:28864531)., Cleaved and activated by CASP1, possibly in the context of inflammation (PubMed:16123779).
- Protein Refseq
- NP_001217.2; NP_116787.1
- Function
- Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis.
Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity).
Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death.
Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity).
Regulates B-cell programs both during early development and after antigen stimulation (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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