Hamster Anti-CD3E Recombinant Antibody (clone 145.2c11) (CAT#: HPAB-A1283-YJ)

Figure 1 Immunoprecipitation analysis of CD3ζ and CD3η proteins in thymic and splenic T-cell subpopulations.

Digitonin lysates of the indicated subpopulations were immunoprecipitated with anti-CD3ε mAb 2C11 and examined by nonreducing SDS/PAGE followed by Western blot analysis using rabbit anti-CD3ζ/η peptide antiserum 389.

Clayton, L. K., D'Adamio, L., Howard, F. D., Sieh, M., Hussey, R. E., Koyasu, S., & Reinherz, E. L. (1991). CD3 eta and CD3 zeta are alternatively spliced products of a common genetic locus and are transcriptionally and/or post-transcriptionally regulated during T-cell development. Proceedings of the National Academy of Sciences, 88(12), 5202-5206.

Figure 2 Flow cytometric analysis.

Parental line 3DO54.8 and transfected line E2 were incubated with anti-human CD3E mAbs Vit3b, Leu4, or RW2-8C8 or with the anti-murine CD3E mAb 2C11.

Transy, C., Moingeon, P., Stebbins, C., & Reinherz, E. L. (1989). Deletion of the cytoplasmic region of the CD3 epsilon subunit does not prevent assembly of a functional T-cell receptor. Proceedings of the National Academy of Sciences, 86(18), 7108-7112.

Figure 3 IL-2 production of E cell lines in response to antigen and crosslinked anti-murine or anti-human CD3e mAb 145.2c11.

Transy, C., Moingeon, P., Stebbins, C., & Reinherz, E. L. (1989). Deletion of the cytoplasmic region of the CD3 epsilon subunit does not prevent assembly of a functional T-cell receptor. Proceedings of the National Academy of Sciences, 86(18), 7108-7112.

Figure 4 Inhibition of antigen-specific lysis of mAb 145.2C11.

Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., & Bluestone, J. A. (1987). Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proceedings of the National Academy of Sciences, 84(5), 1374-1378.