Hamster Anti-CD3E Recombinant Antibody (clone 145.2c11)
CAT#: HPAB-A1283-YJ
This is an IgG antibody that targets the murine CD3e subunit. It is produced by a hybrid cell line created from a combination of hamster and mouse cells. This antibody shows specificity towards the CD3e subunit and exhibits similar reactivity with both the parental and e cell lines.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
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Specifications
- Host Species
- Hamster
- Type
- Hamster IgG
- Specificity
- Mouse CD3E
- Species Reactivity
- Mouse
- Clone
- 145.2c11
- Applications
- ELISA, WB, IP, FC, FuncS, Inhib
- Related Disease
- γδ T cell dysfunction-associated diseases, such as chronic non-healing wounds or cancer.
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the Certificate of Analysis for the lot-specific concentration.
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Enzyme-linked Immunosorbent Assay, Immunoprecitation, Flow Cytometry, Functional Assay, and Inhibition. Optimal working dilutions must be determined by the end user.
Target
- Alternative Names
- T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
- Gene ID
- 916
- UniProt ID
- P07766
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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