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    Rat Anti-CD3E Recombinant Antibody (clone C3-147)

    CAT#: VS-0724-XY71

    The product is a recombinant rat antibody that specifically recognizes human CD3E (CD3 epsilon subunit of T-cell receptor complex), which plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. It has been reported for use in Enzyme-linked Immunosorbent Assay.

    Gene Expression
    ❮
    Figure 1 IF staining of human cell line JURKAT Figure 2 Lymph node Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
    ❯

    Specifications

    • Host Species
    • Rat
    • Type
    • Rat IgG2b
    • Specificity
    • CD3E
    • Species Reactivity
    • Human
    • Clone
    • C3-147
    • Applications
    • ELISA
    • Related Disease
    • Immunodeficiency 18

    Product Property

    • Purification
    • Protein G affinity purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • No preservatives
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Target

    • Alternative Names
    • T3E; TCRE; IMD18; CD3epsilon
    • Cellular Localization
    • Cell membrane
    • Post Translation Modifications
    • Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
      Ubiquitination at Lys104 and Lys177.
    • Function
    • Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.
      When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z.
      All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
      Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
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    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Datasheet

    Datasheet - VS-0724-XY71.pdf

    MSDS

    MSDS - VS-0724-XY71.pdf

    COA

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

    Lot Number:

    Isotype Control

    • CAT
    • Product Name

    Recommended Dilution Buffer

    • CAT
    • Product Name

    Secondary Antibody

    • CAT
    • Product Name

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    Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE
    Download the full case study
    Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE
    Download the full case study

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