Rat Anti-CD3E Recombinant Antibody (clone C3-147) (CAT#: VS-0724-XY71)
The product is a recombinant rat antibody that specifically recognizes human CD3E (CD3 epsilon subunit of T-cell receptor complex), which plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. It has been reported for use in Enzyme-linked Immunosorbent Assay.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Figure 2 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
Specifications
- Host Species
- Rat
- Type
- Rat IgG2b
- Specificity
- CD3E
- Species Reactivity
- Human
- Clone
- C3-147
- Applications
- ELISA
- Related Disease
- Immunodeficiency 18
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target
- Alternative Names
- T3E; TCRE; IMD18; CD3epsilon
- Gene ID
- 916
- UniProt ID
- P07766
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Ubiquitination at Lys104 and Lys177.
- Protein Refseq
- NP_000724.1
- Function
- Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.
When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z.
All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H28 | Anti-Human CD3E Recombinant Antibody (Ertumaxomab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG2a / G2b |
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