Human Anti-CMV gH Recombinant Antibody (clone 3/2) (CAT#: FAMAB-0249JF)
Recombinant monoclonal antibody specially binds to the N-terminal part (AASEALDPHAFHLLLNTYGR) of HVMV gH. This human anti-HCMV gH antibody (3/2) may play an important role in the treatment of CMV infection.
Specific Inquiry
FC
Figure 1 Epithelial cells express adenovirus-encoded CMV glycoproteins on their surfaces.
ARPE-19 cells were transduced with adenovirus encoding either gB or gH/gL or cotransfected with adenoviruses encoding gH/gL.
Fouts, A. E., Chan, P., Stephan, J. P., Vandlen, R., & Feierbach, B. (2012). Antibodies against the gH/gL/UL128/UL130/UL131 complex comprise the majority of the anti-cytomegalovirus (anti-CMV) neutralizing antibody response in CMV hyperimmune globulin. Journal of virology, 86(13), 7444-7447.
ELISA
Figure 2 Neutralization of CMV VR1814 by CMV-HIG, depleted of specific CMV glycoprotein antigens.
To evaluate neutralization potency, depleted CMV-HIG was serially diluted and incubated with virus at an MOI of 1 for 1 h.
Fouts, A. E., Chan, P., Stephan, J. P., Vandlen, R., & Feierbach, B. (2012). Antibodies against the gH/gL/UL128/UL130/UL131 complex comprise the majority of the anti-cytomegalovirus (anti-CMV) neutralizing antibody response in CMV hyperimmune globulin. Journal of virology, 86(13), 7444-7447.
WB
Figure 3 pUL128 and pUL130 are present in virions.
The positions at which marker proteins migrated are identified by their molecular weights (MW).
Wang, D., & Shenk, T. (2005). Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism. Proceedings of the National Academy of Sciences, 102(50), 18153-18158.
IHC
Figure 4 Immunohistochemical detection of HCMV in normal breast and breast cancer.
Negative control (IE isotype control antibody staining for CD34) immunoreactivity is seen only in vascular endothelial cells in an area of infiltrative ductal carcinoma.
Harkins, L. E., Matlaf, L. A., Soroceanu, L., Klemm, K., Britt, W. J., Wang, W., ... & Cobbs, C. S. (2010). Detection of human cytomegalovirus in normal and neoplastic breast epithelium. Herpesviridae, 1(1), 8.
Specifications
- Host Species
- Human
- Derivation
- Human phage display scFv library
- Type
- Human IgG
- Specificity
- CMV gH
- Species Reactivity
- HCMV
- Clone
- 3/2
- Applications
- WB, ELISA, IHC, FC
- Related Disease
- HCMV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CMV gH antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- CMV gH; Cytomegalovirus Glycoprotein H; HHV-5; Human herpesvirus 5; HCMV
- Function
- Neutralizing HCMV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1516CQ | Recombinant Mouse Anti-CMV gH Antibody (0861) | IHC-P, IF, ELISA | Mouse antibody |
| MRO-1517CQ | Recombinant Mouse Anti-CMV gH Antibody (1616580) | ELISA | Mouse antibody |
| MRO-1518CQ | Recombinant Mouse Anti-CMV gH Antibody (5E259) | IF, ELISA | Mouse antibody |
| MRO-1519CQ | Recombinant Mouse Anti-CMV gH Antibody (HCMV16 (51C1)) | IHC, ELISA, FuncS, IF, IP | Mouse antibody |
| FAMAB-0250JF | Human Anti-CMV gH Recombinant Antibody (clone 3/15) | WB, ELISA, IHC | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0249JF-F(E) | Human Anti-CMV gH Recombinant Antibody (clone 3/2); Fab Fragment | ELISA, FuncS | Human Fab |
| FAMAB-0250JF-F(E) | Human Anti-CMV gH Recombinant Antibody (clone 3/15); Fab Fragment | ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0249JF-S(P) | Human Anti-CMV gH Recombinant Antibody (clone 3/2); scFv Fragment | ELISA, FuncS | Human scFv |
| FAMAB-0250JF-S(P) | Human Anti-CMV gH Recombinant Antibody (clone 3/15); scFv Fragment | ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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