Mouse Anti-CysLT Recombinant Antibody (clone 9B12)
CAT#: PABC-502
This product is a recombinant Mouse antibody that can recognize cysLT. Antibody 9B12 binds to LTC4, LTD4, and LTE4, but not to LTB4, 14.15-LTE4, or 5S-HETE.
Published Data
ELISA
Figure 1 Characterization of antibody binding to Cysteinyl Leukotrienes (CysLTs).
Direct competition ELISA of four anti-CysLT antibodies (14H3-black; 9B12-green; 10G4-blue; 2G9-red) binding to native LTC4 and LTD4, respectively.
King, A. N., Fleming, J. K., Knapik, S. S., Visentin, B., Wojciak, J. M., & Huxford, T. (2017). High-affinity pan-specific monoclonal antibodies that target cysteinyl leukotrienes and show efficacy in an acute model of colitis. Journal of lipid research, 58(7), 1386-1398.
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Specifications
- Immunogen
- LTE4 conjugated to protein carriers via disuccinimidyl homobifunctional cross-linking
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Clone
- 9B12
- Applications
- ELISA
- Related Disease
- Inflammatory diseases and asthma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- CysLT; Cysteinyl leukotriene
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Easy to Handle
Handling this antibody was simple and user-friendly. The instructions were clear, and the performance was consistently high, making it a dependable reagent.
Tailored Blocking Recommendations
Following the blocking recommendations provided, I achieved minimal background noise and high signal specificity. The antibody works very well with the suggested blocking agents.
Superb for Signal Detection
The signal detection using this antibody was superb. It provided clear and strong signals, making the analysis effectively simple and precise.
Q&As
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How do personal comments affect antibody performance?
A: Personal handling procedures, such as pipetting and buffer preparation, can affect antibody performance and reproducibility.
-
Should antibodies from new suppliers be validated?
A: Validating novel antibodies from several providers ensures that they perform consistently in your specific experimental setup.
-
What are the "housekeeping" antibodies?
A: Housekeeping antibodies target proteins that are routinely produced at stable quantities and serve as loading controls in applications such as Western blots.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 9B12"
- CAT
- Product Name
See other products for "CysLT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-497 | Human Anti-CysLT Recombinant Antibody (clone LT5014/Hu10G4) | ELISA | Humanized IgG1, κ |
| PABC-498 | Mouse Anti-CysLT Recombinant Antibody (clone 10G4) | ELISA | Mouse IgG1, κ |
| PABC-499 | Mouse Anti-CysLT Recombinant Antibody (clone 14H3) | ELISA | Mouse IgG1, κ |
| PABC-500 | Mouse Anti-CysLT Recombinant Antibody (clone 2F9) | ELISA | Mouse IgG1, κ |
| PABC-503 | Mouse Anti-CysLT Recombinant Antibody (clone 2G9) | ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-497 | Human Anti-CysLT Recombinant Antibody (clone LT5014/Hu10G4); Fab Fragment | ELISA | Humanized Fab |
| PFBC-498 | Mouse Anti-CysLT Recombinant Antibody (clone 10G4); Fab Fragment | ELISA | Mouse Fab |
| PFBC-499 | Mouse Anti-CysLT Recombinant Antibody (clone 14H3); Fab Fragment | ELISA | Mouse Fab |
| PFBC-500 | Mouse Anti-CysLT Recombinant Antibody (clone 2F9); Fab Fragment | ELISA | Mouse Fab |
| PFBC-502 | Mouse Anti-CysLT Recombinant Antibody (clone 9B12); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-497 | Human Anti-CysLT Recombinant Antibody (clone LT5014/Hu10G4); scFv Fragment | ELISA | Humanized scFv |
| PSBC-498 | Mouse Anti-CysLT Recombinant Antibody (clone 10G4); scFv Fragment | ELISA | Mouse scFv |
| PSBC-499 | Mouse Anti-CysLT Recombinant Antibody (clone 14H3); scFv Fragment | ELISA | Mouse scFv |
| PSBC-500 | Mouse Anti-CysLT Recombinant Antibody (clone 2F9); scFv Fragment | ELISA | Mouse scFv |
| PSBC-502 | Mouse Anti-CysLT Recombinant Antibody (clone 9B12); scFv Fragment | ELISA | Mouse scFv |
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