Mouse Anti-CysLT Recombinant Antibody (clone 9B12) (CAT#: PABC-502)
This product is a recombinant Mouse antibody that can recognize cysLT. Antibody 9B12 binds to LTC4, LTD4, and LTE4, but not to LTB4, 14.15-LTE4, or 5S-HETE.
Specific Inquiry
ELISA
Figure 1 Characterization of antibody binding to Cysteinyl Leukotrienes (CysLTs).
Direct competition ELISA of four anti-CysLT antibodies (14H3-black; 9B12-green; 10G4-blue; 2G9-red) binding to native LTC4 and LTD4, respectively.
King, A. N., Fleming, J. K., Knapik, S. S., Visentin, B., Wojciak, J. M., & Huxford, T. (2017). High-affinity pan-specific monoclonal antibodies that target cysteinyl leukotrienes and show efficacy in an acute model of colitis. Journal of lipid research, 58(7), 1386-1398.
Specifications
- Immunogen
- LTE4 conjugated to protein carriers via disuccinimidyl homobifunctional cross-linking
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Clone
- 9B12
- Applications
- ELISA
- Related Disease
- Inflammatory diseases and asthma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- CysLT; Cysteinyl leukotriene
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-497 | Human Anti-CysLT Recombinant Antibody (clone LT5014/Hu10G4) | ELISA | Humanized IgG1, κ |
| PABC-498 | Mouse Anti-CysLT Recombinant Antibody (clone 10G4) | ELISA | Mouse IgG1, κ |
| PABC-499 | Mouse Anti-CysLT Recombinant Antibody (clone 14H3) | ELISA | Mouse IgG1, κ |
| PABC-500 | Mouse Anti-CysLT Recombinant Antibody (clone 2F9) | ELISA | Mouse IgG1, κ |
| PABC-503 | Mouse Anti-CysLT Recombinant Antibody (clone 2G9) | ELISA | Mouse IgG1, κ |
Customer Reviews and Q&As
Customer Review
Q&As
Easy to Handle
Handling this antibody was simple and user-friendly. The instructions were clear, and the performance was consistently high, making it a dependable reagent.
Tailored Blocking Recommendations
Following the blocking recommendations provided, I achieved minimal background noise and high signal specificity. The antibody works very well with the suggested blocking agents.
Superb for Signal Detection
The signal detection using this antibody was superb. It provided clear and strong signals, making the analysis effectively simple and precise.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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