Rabbit Anti-DDR1 (p796) Recombinant Antibody (clone 20G11) (CAT#: HPAB-N0384-YC)
Provided is a monoclonal antibody specifically binds to the discoidin domain receptor 1 (DDR1) which is phosphorylated at the tyrosine in position 796 of the DDR1 sequence.
Specific Inquiry
IHC
Figure 1 Detection of pDDRl in IHC FFPE control cell lines.
Phosphorylation was induced by stimulation of the U20S cells with collagen. Subsequent to stimulation the cells were fixed and paraffin embedded. It is shown that the selected clones can detect phosphorylated DDR1 (lower panel), whereas no signal is obvious in the unstimulated control cells (upper panel). Membrane staining of phosphorylated DDR1 is highlighted with black arrows (lower panel).
WB
Figure 2 Detection of DDR1 and pDDRl, respectively, by Western Blotting.
U20S cells were treated with collagen to stimulate the cells and to induce DDR1 phosphorylation. For the negative control lysates, untreated U20S cells were used. Subsequent to stimulation the cells were lyzed, subjected to SDS-PAGE, transferred to nitrocellulose and analyzed for presence of DDR1 and pDDRl, respectively by probing with the antibodies depicted in the Figure.
Specifications
- Immunogen
- The peptide DDRl_HUMAN (788-808) [pY796] coupled to KLH
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DDR1 (p796)
- Species Reactivity
- Human
- Clone
- 20G11
- Applications
- IHC, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry and Western Blot. Please see the Published Data for further information.
Target
- Alternative Names
- CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
- Gene ID
- 780
- UniProt ID
- Q08345
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0384-YC-S(P) | Rabbit Anti-DDR1 (p796); scFv Fragment Recombinant Antibody (clone 20G11); scFv Fragment | IHC, WB | Rabbit scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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