Recombinant Mouse Anti-DENV2 ED3 Antibody (3H5)
CAT#: PABC-310
This product is a recombinant Mouse antibody that can recognize DENV2 ED3. Antibody 3H5 is highly unusual as it not only is potently neutralizing but also promotes little if any ADE.
Published Data
Tested Data
IF
Figure 5 DENV2 E antigen was detected in testis and peripheral tissue of accessory glands by IFA using DENV2 specific 3H5 monoclonal antibody.
Sánchez-Vargas, I., Harrington, L. C., Doty, J. B., Black 4th, W. C., & Olson, K. E. (2018). Demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of Aedes aegypti. PLoS neglected tropical diseases, 12(8), e0006754.
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SDS-PAGE
Figure 1 Recombinant Mouse Anti-DENV2 ED3 Antibody (PABC-310) in SDS-PAGE
Lane 1: Non-reduced mAb
Lane 2: Reduced mAb
SEC-HPLC
Figure 2 Recombinant Mouse Anti-DENV2 ED3 Antibody (PABC-310) in HPLC
The purity of PABC-310 was greater than 95% as determined by SEC-HPLC.
ELISA
Figure 3 Recombinant Mouse Anti-DENV2 ED3 Antibody (PABC-310) in ELISA
ELISA analysis of PABC-310 was performed by coating withDengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 4 Recombinant Mouse Anti-DENV2 ED3 Antibody (PABC-310) in WB
Western blot analysis of PABC-310 was performed by loading Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (ECD, His Tag).
PABC-310 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- DENV2 ED3
- Species Reactivity
- DENV-2
- Clone
- 3H5
- Applications
- ELISA, WB, IF, IP, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The antibody 3H5 has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation, Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- DENV2 ED3; Dengue virustype 2 ED3; Domain III of Dengue virus 2
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 3H5"
- CAT
- Product Name
See other products for "DENV2 ED3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-279 | Recombinant Mouse Anti-DENV2 ED3 Antibody (2C8) | ELISA, DB, WB, IF, IP, Neut | Mouse IgG |
| PABC-418 | Recombinant Human Anti-DENV2 ED3 Antibody (h3H5) | ELISA, WB, IF, IP, Neut | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-279 | Recombinant Mouse Anti-DENV2 ED3 Antibody Fab Fragment (2C8) | ELISA, WB, IF, IP, Neut | Mouse Fab |
| PFBC-310 | Recombinant Mouse Anti-DENV2 ED3 Antibody Fab Fragment (3H5) | ELISA, WB, IF, IP, Neut | Mouse Fab |
| PFBC-418 | Recombinant Human Anti-DENV2 ED3 Antibody Fab Fragment (h3H5) | ELISA, WB, IF, IP, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-279 | Recombinant Mouse Anti-DENV2 ED3 Antibody scFv Fragment (2C8) | ELISA, WB, IF, IP, Neut | Mouse scFv |
| PSBC-310 | Recombinant Mouse Anti-DENV2 ED3 Antibody scFv Fragment (3H5) | ELISA, WB, IF, IP, Neut | Mouse scFv |
| PSBC-418 | Recombinant Human Anti-DENV2 ED3 Antibody scFv Fragment (h3H5) | ELISA, WB, IF, IP, Neut | Human scFv |
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