Recombinant Human Anti-DENV2 ED3 Antibody (h3H5) (CAT#: PABC-418)
This product is a recombinant Human antibody that can recognize DENV2 ED3. Antibody 3H5 is highly unusual as it not only is potently neutralizing but also promotes little if any ADE.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Binding specificity of D29 Fab-IgG.
To determine the binding specificity of D29 Fab-IgG in a sandwich ELISA format, DENV2 in infected Vero cell culture media was captured by immobilized m4G2 and detected by 1 mg/ml D29 Fab-IgG h3H5 and h4G2.
Chan, A. H. Y., Tan, H. C., Chow, A. Y., Lim, A. P. C., Lok, S. M., Moreland, N. J., ... & Hanson, B. J. (2012). A human PrM antibody that recognizes a novel cryptic epitope on dengue E glycoprotein. PLoS One, 7(4), e33451.
IP
Figure 2 Immunoprecipitation of DENV2 proteins with antiDENV antibodies.
For immunoprecipitation with 1.25% SDS, cleared lysate of DENV2-infected BHK cells were incubated with h3H5, h4G2, m2H2, D29 Fab-IgG or non-DENV specific human antibody (IgG) followed by analysis with silver staining and Western blotting. Precipitated prM and E proteins were verified by (anti-prM) HRP-conjugated m2H2 or (anti-E) h3H5 respectively.
Chan, A. H. Y., Tan, H. C., Chow, A. Y., Lim, A. P. C., Lok, S. M., Moreland, N. J., ... & Hanson, B. J. (2012). A human PrM antibody that recognizes a novel cryptic epitope on dengue E glycoprotein. PLoS One, 7(4), e33451.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG1
- Specificity
- DENV2 ED3
- Species Reactivity
- DENV-2
- Clone
- h3H5
- Applications
- ELISA, WB, IF, IP, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- DENV2 ED3; Dengue virustype 2 ED3; Domain III of Dengue virus 2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-279 | Recombinant Mouse Anti-DENV2 ED3 Antibody (2C8) | ELISA, DB, WB, IF, IP, Neut | Mouse IgG |
| PABC-310 | Recombinant Mouse Anti-DENV2 ED3 Antibody (3H5) | ELISA, WB, IF, IP, Neut | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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