Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC07-αCD3) (CAT#: MRO-3273CQ)

Figure 1 Bispecific immunomodulatory protein binds the CD4-binding site (CD4bs) of HIV Env and CD3.

The indicated immunodulatory bispecific and control proteins were allowed to bind to naive human T cells and any protein bound to the T cells was detected with flow cytometry after dual staining with fluorescently labelled anti-human Fab and HIV-1 Env (RSC3) probes.

Pegu, A., Asokan, M., Wu, L., Wang, K., Hataye, J., Casazza, J. P., ... & Chen, X. (2015). Activation and lysis of human CD4 cells latently infected with HIV-1. Nature communications, 6(1), 1-9.

Figure 2 Activation and targeted lysis of chronic and latent HIV-infected cells by VRC07-αCD3.

Induction of HIV in latent cell lines. A chronic cell line (CEM-IIIb) was cultured in the absence or presence of TNF-α for 14-16 h and the expression of HIV Env onthe cell surface using an allophycocyanin (APC)-conjugated 2G12 was measured using flow cytometry. The increase in the expression of HIV Env indicates the inducible expression in the latent cell lines compared with the constitutive expression in the chronic cell line.

Pegu, A., Asokan, M., Wu, L., Wang, K., Hataye, J., Casazza, J. P., ... & Chen, X. (2015). Activation and lysis of human CD4 cells latently infected with HIV-1. Nature communications, 6(1), 1-9.