Human Anti-HIV-1 Tat Recombinant Antibody (clone J1) (CAT#: MRO-4313CQ)
Recombinant human antibody to Tat. J1 binds strongly to soluble rTAT protein and to denatured rTAT, suggesting that the epitope is linear. J1 recognized HIV-1 clade B Tat proteins Bru and HXB2, but did not bind to clade E (CM240), clade C (92Br), clade D.
Specific Inquiry
WB
Figure 1 Western blotting was detected by antiserum from a Tat-immunized scFv J1.
SDS-PAGE and Western blotting
were carried out, and immunodetection was performed using antiserum from a Tat-immunized rabbit.
Moreau, E., Hoebeke, J., Zagury, D., Muller, S., & Desgranges, C. (2004). Generation and characterization of neutralizing human monoclonal antibodies against human immunodeficiency virus type 1 Tat antigen. Journal of virology, 78(7), 3792-3796.
Neut
Figure 2 LTR-dependent CAT production by HL3T1 cells was measured after preincubation of active rTat with increasing concentrations of antibodies.
After coculture with CEM cells transiently expressing native Tat 1-72 in the
presence of fixed amounts of antibodies.
Moreau, E., Hoebeke, J., Zagury, D., Muller, S., & Desgranges, C. (2004). Generation and characterization of neutralizing human monoclonal antibodies against human immunodeficiency virus type 1 Tat antigen. Journal of virology, 78(7), 3792-3796.
Specifications
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- Recognizes HIV-1 Tat
- Species Reactivity
- HIV-1
- Clone
- J1
- Applications
- Neut, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Tat antibody has been reported in applications of Western Blot, Neutralization.
Target
- Alternative Names
- TAT; p14; Tat; Transactivating regulatory protein; HIV-1
- Gene ID
- 155871
- UniProt ID
- P04608
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone J1"
See other products for "HIV-1 Tat"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-565 | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 11H6H1); Fab Fragment | ELISA, Neut | Mouse Fab |
| MRO-4310CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone B1E3); Fab Fragment | ELISA, Neut | Human Fab |
| MRO-4311CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone G1); Fab Fragment | WB, IP, Neut | Human Fab |
| MRO-4312CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone G2); Fab Fragment | WB, IP, Neut | Human Fab |
| MRO-4313CQ-F(E) | Human Anti-HIV-1 Tat Recombinant Antibody (clone J1); Fab Fragment | WB, IP, Neut | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-565 | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 11H6H1); scFv Fragment | ELISA, Neut | Mouse scFv |
| MRO-4310CQ-S(P) | Human Anti-HIV-1 Tat Recombinant Antibody (clone B1E3); scFv Fragment | ELISA, Neut | Human scFv |
| MRO-4311CQ-S(P) | Human Anti-HIV-1 Tat Recombinant Antibody (clone G1); scFv Fragment | WB, IP, Neut | Human scFv |
| MRO-4312CQ-S(P) | Human Anti-HIV-1 Tat Recombinant Antibody (clone G2); scFv Fragment | WB, IP, Neut | Human scFv |
| MRO-4313CQ-S(P) | Human Anti-HIV-1 Tat Recombinant Antibody (clone J1); scFv Fragment | WB, IP, Neut | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1023CQ | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 02-001) | WB, IP | Mouse IgG2b |
| MRO-1024CQ | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 02-002) | WB, IP | Mouse IgG1 |
| MRO-1025CQ | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 02-010) | WB, IP | Mouse IgM |
| MRO-1026CQ | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 02-012) | WB, IP | Mouse IgG1 |
| MRO-1027CQ | Mouse Anti-HIV-1 Tat Recombinant Antibody (clone 1) | RIPA, IHC-P, IHC-Fr, ELISA, WB | Mouse IgG1 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for MRO-4313CQ. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.