Mouse Anti-HIV CD4bs Recombinant Antibody (clone B-p1b5) (CAT#: FAMAB-0189WJ)
Provided is a HIV-1 neutralizing antibody (clone B-p1b5) that targets the CD4-binding site of Env. Both 426c WT Core and HxB2 WT Core could be immunoprecipitated by B-p1b5. B-p1b5 neutralized the WT 426c virus (IC50 of 32.05 μg/mL) expressed in GnTI−/− cells.
Specific Inquiry
Figure 1 Trimeric Env-Binding and Neutralizing Properties of VRC01-like Antibodies Elicited by the 426c Core Germline-Targeting Immunogen.
The binding of eight VRC01-like antibodies generated following the prime immunization against (A) the autologous 426c WT DS-SOSIP; (B) its variant that lacks the N276, N460, and N463 NLGS (426c DS-SOSIP D3); (C) a variant that only lacks the N276 glycan (426c D276 DS-SOSIP); and (D) the heterologous 45_01dG5 NFL TD-2CC+(DS+), which also lacks the N276 NLGS, was measured using Biolayer interferometry.
Parks, K. R., MacCamy, A. J., Trichka, J., Gray, M., Weidle, C., Borst, A. J., Khechaduri, A., Takushi, B., Agrawal, P., Guenaga, J., Wyatt, R. T., Coler, R., Seaman, M., LaBranche, C., Montefiori, D. C., Veesler, D., Pancera, M., McGuire, A., & Stamatatos, L. (2019). Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization. Cell reports, 29(10), 3060–3072.e7.
Co-IP
Figure 2 Vaccine Elicited Antibodies Bind in to Env Core with Glycans at N276.
Following co-immunoprecipitation, 426c WT Core (left) and HxB2 WT Core (right) were washed and eluted from the beads.
Parks, K. R., MacCamy, A. J., Trichka, J., Gray, M., Weidle, C., Borst, A. J., Khechaduri, A., Takushi, B., Agrawal, P., Guenaga, J., Wyatt, R. T., Coler, R., Seaman, M., LaBranche, C., Montefiori, D. C., Veesler, D., Pancera, M., McGuire, A., & Stamatatos, L. (2019). Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization. Cell reports, 29(10), 3060–3072.e7.
Specifications
- Immunogen
- 426c Core Env
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG
- Specificity
- CD4-binding site of Env
- Species Reactivity
- HIV-1
- Clone
- B-p1b5
- Applications
- ELISA, WB, Neut
- Related Disease
- HIV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Human immunodeficiency virus; HIV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0178WJ | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P6) | ELISA, WB, Neut | Human IgG |
| FAMAB-0179WJ | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P11) | ELISA, WB, Neut | Human IgG |
| FAMAB-0180WJ | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P9) | ELISA, WB, Neut | Human IgG |
| FAMAB-0181WJ | Human Anti-HIV CD4bs Recombinant Antibody (clone N60P1.1) | ELISA, WB, Neut | Human IgG |
| FAMAB-0182WJ | Mouse Anti-HIV CD4bs Recombinant Antibody (clone P-p1e7) | ELISA, WB, Neut | Mouse IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0178WJ-S(P) | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P6); scFv Fragment | ELISA, WB, Neut | Human scFv |
| FAMAB-0179WJ-S(P) | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P11); scFv Fragment | ELISA, WB, Neut | Human scFv |
| FAMAB-0180WJ-S(P) | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P9); scFv Fragment | ELISA, WB, Neut | Human scFv |
| FAMAB-0181WJ-S(P) | Human Anti-HIV CD4bs Recombinant Antibody (clone N60P1.1); scFv Fragment | ELISA, WB, Neut | Human scFv |
| FAMAB-0182WJ-S(P) | Mouse Anti-HIV CD4bs Recombinant Antibody (clone P-p1e7); scFv Fragment | ELISA, WB, Neut | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0178WJ-F(E) | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P6); Fab Fragment | ELISA, WB, Neut | Human Fab |
| FAMAB-0179WJ-F(E) | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P11); Fab Fragment | ELISA, WB, Neut | Human Fab |
| FAMAB-0180WJ-F(E) | Human Anti-HIV CD4bs Recombinant Antibody (clone N49P9); Fab Fragment | ELISA, WB, Neut | Human Fab |
| FAMAB-0181WJ-F(E) | Human Anti-HIV CD4bs Recombinant Antibody (clone N60P1.1); Fab Fragment | ELISA, WB, Neut | Human Fab |
| FAMAB-0182WJ-F(E) | Mouse Anti-HIV CD4bs Recombinant Antibody (clone P-p1e7); Fab Fragment | ELISA, WB, Neut | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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