Mouse Anti-Igδ2 Recombinant Antibody (clone 7D11) (CAT#: FAMAB-0026-CN)

• Immunogen
• E. coli-expressed rIgδ2 protein

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Catfish Igδ2

• Species Reactivity
• Catfish

• Clone
• 7D11

• Applications
• IP, FC

• Purity
• >95% as determined by SDS-PAGE and HPLC analysis

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Western Blot, Immunoprecipitation and Flow Cytometry.
  FC: for flow-cytometry analyses, aliquots of 1×10⁶ PBLs or sorted populations were incubated with 50 ml mAb supernatant for 30 min at 4˚C, washed, and incubated with 50 ml goat anti-mouse secondary Abs conjugated to PE diluted 1:80 (v/v) in cfRPMI or FITC diluted 1:40 (v/v) in cfRPMI for 30 min at 4˚C. After washing, cells were suspended in 0.3 ml cfRPMI and analyzed for single or two-color staining by flow cytometry.
  IP: Immunoprecipitations were performed using Sepharose beads that were prepared in a stepwise fashion. First, 1 g lyophilized cyanogen bromide (CNBr)-activated Sepharose 4B beads was washed in 200 ml 1 mM HCl and resuspended in 5 ml coupling buffer containing 0.5 mg goat anti-mouse IgM for 1 h. After washing in coupling buffer, any remaining active groups were blocked by incubating the coupled beads in 0.1 M Tris-HCl (pH 8) for 2 h, and the beads were washed in alternating high- and low-pH buffers to remove excess uncoupled ligand. Second, 8 ml anti-catfish Igd mAb 7D11 hybridoma supernatant, diluted 1:1 in low-stringency buffer (100 mM NaCl, 20 mM Tris-HCl [pH 7.7]), was added to the goat antimouse IgM-coupled beads, which were allowed to incubate with end-toend rotation overnight at 4˚C. After incubation, the beads were washed in low-stringency buffer (total of 30 ml added in six aliquots) and resuspended in 4 ml low-stringency buffer.

• Alternative Names
• Igδ2

• FAMAB-0026-CN-S(P)
• Mouse Anti-Igδ2 Recombinant Antibody (clone 7D11); scFv Fragment

• FAMAB-0026-CN-F(E)
• Mouse Anti-Igδ2 Recombinant Antibody (clone 7D11); Fab Fragment

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