Mouse Anti-KDM6A Recombinant Antibody (clone 6E2D6) (CAT#: VS7-0425-WR206)
This product is a mouse IgG2a antibody that binds to human KDM6A. The antibody 6E2D6 recognizes specific amino acid domain of human KDM6A (AA: 1252-1401). It can be used for WB assay. It cross reacts with rat.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Normal Tissue
Subcellular Location
RNA Expression
Figure 1 Bone marrow
(High expression in bone marrow)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_4_4_val_selected.jpg
(High expression in bone marrow)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_4_4_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_B_8_5.jpg
Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_9_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_9_3.jpg
Figure 5 Placenta
(Cytotrophoblasts Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity: 75%-25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_3_7.jpg
(Cytotrophoblasts Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity: 75%-25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_3_7.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2111/10157_A_8_8.jpg
Figure 2 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/568/1858_B11_32_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/568/1858_B11_32_selected.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147050-KDM6A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147050-KDM6A
Specifications
- Immunogen
- Recombinant human KDM6A (AA: 1252-1401) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human KDM6A
- Species Reactivity
- Human, Rat
- Clone
- 6E2D6
- Molecule Class
- Demethylase
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Kabuki syndrome 2 (KABUK2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KDM6A; BA386N14.2; EC 1.14.11; Ubiquitously-Transcribed X Chromosome Tetratricopeptide Repeat Protein; Ubiquitously-Transcribed TPR Protein On The X Chromosome; Ubiquitously Transcribed Tetratricopeptide Repeat, X Chromosome; Ubiquitously-Transcribed TPR
- Gene ID
- 7403
- UniProt ID
- O15550
- Long Name
- Lysine demethylase 6A
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Methylation; Phosphoprotein
- Protein Refseq
- NP_001278344.1
- Function
- Chromatin regulator; Dioxygenase; Oxidoreductase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3996z | Mouse Anti-KDM6A Recombinant Antibody (clone 26G12) | WB, ELISA, FC, ICC, IHC | Mouse IgG2a |
| MOB-0105CT | Recombinant Mouse anti-Human KDM6A Monoclonal antibody (4B3) | ELISA, IP, WB | |
| VS3-CJ42 | Mouse Anti-KDM6A Recombinant Antibody (VS3-CJ42) | WB, ELISA | Mouse IgG |
| VS7-0425-WR205 | Mouse Anti-KDM6A Recombinant Antibody (clone 5C2A9) | FC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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