Mouse Anti-LIPE Recombinant Antibody (VS3-FY2614)
CAT#: VS3-FY2614
This anti-HSL mAb is a mouse recombinant antibody that is created to recognize lipase E, hormone sensitive type, a calculated weight of 116 kDa. It exhibits cross-reactivity with human and mouse. This monoclonal antibody is potential for conducting research on Lipodystrophy, Familial Partial, Type 6 and Familial Partial Lipodystrophy. The antibody can be utilized for detection of HSL by Western Blot, Immunocytochemistry, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6567/1740_C2_18_cr57fb5aceee28e_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6567/1657_A6_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17700/132029_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17700/132029_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17700/132029_A_6_6.jpg
Normal Tissue
Figure 6 Breast
Adipocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17700/132029_B_2_4.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17700/132029_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (ASC diff)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079435-LIPE
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- lipase E, hormone sensitive type of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 116 kDa.
- Related Disease
- Lipodystrophy, Familial Partial, Type 6 and Familial Partial Lipodystrophy
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunocytochemistry: 1/50-1/100
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- HSL; LHS; REH; AOMS4; FPLD6
- Gene ID
- 3991
- Sequence Similarities
- Belongs to the 'GDXG' lipolytic enzyme family.
- Cellular Localization
- Cell membrane
Membrane, caveola
Cytoplasm, cytosol
Lipid droplet
- Post Translation Modifications
- Phosphorylation by AMPK reduces its translocation towards the lipid droplets.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- XP_005258994.1; XP_005258996.2; XP_006723281.1; XP_047294791.1; XP_047294792.1
- Function
- Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:8812477, 15955102, 15716583, 19800417).
Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs.
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Related Diseases
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0010JF | Mouse Anti-HSL Recombinant Antibody (clone HSL-2) | WB, ELISA, IHC, FC | Chimeric (sheep/mouse) IgG1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0010JF-F(E) | Mouse Anti-HSL Recombinant Antibody (clone HSL-2); Fab Fragment | WB, ELISA, FuncS | Chimeric (sheep/mouse) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0010JF-S(P) | Sheep Anti-HSL Recombinant Antibody (clone HSL-2); scFv Fragment | WB, ELISA, FuncS | Sheep scFv |
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