Mouse Anti-HSL Recombinant Antibody (clone HSL-2) (CAT#: FAMAB-0010JF)
Recombinant monoclonal antibody can sepecially bind to HSL. This chimeric ( sheep/mouse) anti-HSL antibody HSL-2 MAb is suitable for the diagnosis, prevention,and treatment of life-threatening bacterial infections.
Specific Inquiry
WB
Figure 1 In vitro validation of phosphospecific HSL antibodies.
Purified recombinant HSL protein was incubated alone or in the presence of PKA or AMPK.
Kraemer, F. B., Patel, S. H. A. I. L. J. A., Saedi, M. S., & Sztalryd, C. (1993). Detection of hormone-sensitive lipase in various tissues. I. Expression of an HSL/bacterial fusion protein and generation of anti-HSL antibodies. Journal of lipid research, 34(4), 663-671.
IHC
Figure 2 Detection of HSL by immunohistochemical staining in solid tumors.
Representative results from one of two independent experiments are shown.
Beck, W. T., Grogan, T. M., Willman, C. L., Cordon-Cardo, C., Parham, D. M., Kuttesch, J. F., ... & Brophy, N. A. (1996). Methods to detect P-glycoprotein-associated multidrug resistance in patients' tumors: consensus recommendations. Cancer research, 56(13), 3010-3020.
ELISA
Figure 3 Standard curves for the competitive ELISA detecting 3oxoC12-HSL and 3oxoC12-HS.
Data are representative of at least 3 independent experiments.
Rothballer, M., Uhl, J., Kunze, J., Schmitt-Kopplin, P., & Hartmann, A. (2018). Detection of the bacterial quorum-sensing signaling molecules N-acyl-homoserine lactones (HSL) and N-acyl-homoserine (HS) with an enzyme-linked immunosorbent assay (ELISA) and via ultrahigh-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS). In Quorum Sensing (pp. 61-72). Humana Press, New York, NY.
IF
Figure 4 Effect of HSL genotype on histology of maximal cell diameter.
The bars in the left panels correspond to 100 um.
Fortier, M., Soni, K., Laurin, N., Wang, S. P., Mauriège, P., Jirik, F. R., & Mitchell, G. A. (2005). Human hormone-sensitive lipase (HSL): expression in white fat corrects the white adipose phenotype of HSL-deficient mice. Journal of lipid research, 46(9), 1860-1867.
Specifications
- Immunogen
- A mixture of three HSL-bovine thyroglobulin conjugates—N-acyl-C12-HSL-TG, 3-oxo-C12-HSL-TG, and 3-OH-C12-HSL-TG
- Host Species
- Mouse
- Derivation
- Chimeric (sheep/mouse)
- Type
- Chimeric (sheep/mouse) IgG1, λ
- Specificity
- Homoserine lactone
- Clone
- HSL-2
- Applications
- WB, ELISA, IHC, FC
- Related Disease
- Lethal Pseudomonas aeruginosa infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HSL antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- HSL; Homoserine lactone
Related Resources
Insulin Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0010JF-F(E) | Mouse Anti-HSL Recombinant Antibody (clone HSL-2); Fab Fragment | WB, ELISA, FuncS | Chimeric (sheep/mouse) Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0010JF-S(P) | Sheep Anti-HSL Recombinant Antibody (clone HSL-2); scFv Fragment | WB, ELISA, FuncS | Sheep scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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