Mouse Anti-LMNB2 Recombinant Antibody (clone 2E2F4) (CAT#: VS7-0425-WR304)
This product is a mouse IgG2b antibody that binds to human LMNB2. The antibody 2E2F4 recognizes specific amino acid domain of human LMNB2 (AA: 401-600). It can be used for WB, IHC, ICC, and FC assays.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/1227_H10_3_selected.jpg

(Immunohistochemical staining of human cerebral cortex shows strong positivity in nuclei and nuclear membranes of neurons.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22593/ihc_selected.jpg

(High expression. RNA expression: 17.3 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/143764_B_4_4_val_selected.jpg

(Low expression. RNA expression: 3.6 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/143764_A_3_8_val_selected.jpg

(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/143764_A_9_3.jpg

(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/143764_A_9_4.jpg

(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/143764_A_9_5.jpg

(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/143764_A_6_6.jpg

(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47863/134959_A_3_6.jpg

(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62477/143764_A_8_8.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176619-LMNB2
Specifications
- Immunogen
- Recombinant human LMNB2 (AA: 401-600) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human LMNB2
- Species Reactivity
- Human
- Clone
- 2E2F4
- Molecule Class
- B-Type Nuclear Lamin Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Partial acquired lipodystrophy (APLD); Epilepsy, progressive myoclonic 9 (EPM9); Microcephaly 27, primary, autosomal dominant (MCPH27)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- EPM9; LMN2; LAMB2; MCPH27
- Gene ID
- 84823
- UniProt ID
- Q03252
- Long Name
- Lamin B2
- Cellular Localization
- Intermediate filament; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Isopeptide bond; Lipoprotein; Methylation; Phosphoprotein; Prenylation; Ubl conjugation
- Protein Refseq
- NP_116126.3
- Function
- Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:33033404).; Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:33033404).; The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:33033404).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "LMNB2"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2069 | Hi-Affi™ Recombinant Rabbit Anti-LMNB2 Monoclonal Antibody (DS2069AB) | WB, FC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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