Mouse Anti-Ln-5 γ2 Recombinant Antibody (clone 5D5) (CAT#: HPAB-1876-FY)

Figure 1 Illustrates the mapping of an ADR for mAb 4G1 (Ln-5 394-414) by direct ELISA.

The results show binding of the 4G1 antibody to the epitope located at amino acid residues 394-414. Two other antibodies, 6C12 and 5D5, do not recognize the same epitope.

Figure 2 Shows the results of a competition ELISA.

The data show that both, mAb 5D5 and mAb 6C12, recognize a complex structural epitope, since all three peptides can to some degree inhibit binding of the antibody to GST-LN5 γIII. However, the epitopes are somewhat different for 5D5 and 6C12, as the peptide 518-537 does not interfere with binding of 5D5, while it inhibits 6C12 binding.

Figure 3 Provides a graph of the results of in vivo metastasis assays performed with mAbs 5D5 and 6C12.

Demonstrating the ability of these antibodies to inhibit metastases in vivo.

Figure 4 Show reflection/refractive index data obtained by surface plasmon resonance affinity assays of mAb 5D5 and a γ2-associated peptide.

Figure 5 Show reflection/refractive index data obtained by surface plasmon resonance affinity assays of mAb 5D5 and a γ2-associated peptide.