Rabbit Anti-LY9 Recombinant Antibody (clone R02-1I0)
CAT#: VS3-FY1985
The anti-CD229 antibody detected in western blot analysis of CD229 in Hela, A549, HL-60, U251 lysates. The recombinant monoclonal antibody demonstrated a greater affinity with human LY9. Western blot demonstrated that R02-1I0 recognized a target protein with a molecular weight of 72 kDa.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/1970_E8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/121038_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/121038_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/121038_A_7_4.jpg
Normal Tissue
Figure 6 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/121038_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/121038_A_9_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50917/121038_A_4_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (Daudi, JURKAT, Karpas-707, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000122224-LY9
❮
❯
WB
Figure 1 Anti-LY9 Antibody (VS3-FY1985) in WB.
The CD229 expression in Hela, A549, HL-60, U251 lysates were detected using the anti-CD229 by western blot assay.
❮
❯
Specifications
- Immunogen
- Recombinant human CD229 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LY9
- Species Reactivity
- Human
- Clone
- R02-1I0
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 72 kDa, while the observed molecular weight stands at 72 kDa.
- Related Disease
- Multisystem Inflammatory Syndrome In Children and Lymphoproliferative Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- LY9; CD229; SLAMF3; hly9; mlymphocyte antigen 9
- Gene ID
- 4063
- UniProt ID
- Q9HBG7
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Glycosylation at Asn68, Asn95, Asn120, Asn169, Asn173, Asn285, Asn413, and Asn424 (NX_Q9HBG7 [NX_Q9HBG7-1])
Modification sites at PhosphoSitePlus (Q9HBG7)
Glycosylation from GlyGen (Q9HBG7) 10 sites, 2 O-linked glycans (1 site)
- Protein Refseq
- XP_011507851.1; XP_011507858.1; XP_011507862.1; XP_016856788.1; XP_016856790.1
- Function
- Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1985. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LY9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0285CT | Recombinant Mouse anti-Human LY9 Monoclonal antibody (IMz0.36) | FC, FuncS, ICC, IP, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY94 | Human Anti-LY9 scFv-Fc Chimera (VS-0325-FY94) | IA | Human IgG1, scFv-Fc |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, WB, BLI, SPR
Application: ELISA, FC, IF, WB
Application: Block, IP, IF, FC
Application: ELISA, Inhib, FuncS
-2.png)
Application: ELISA, WB
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
