Mouse Anti-NFAT5 Recombinant Antibody (clone 1D6)
CAT#: VS3-WK1588
This antibody is a mouse recombinant antibody against human NFAT5, which is a member of the nuclear factors of activated T cells family of transcription factors. The clone 1D6 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57304/991_C2_2_selected.jpg
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Specifications
- Immunogen
- Synthesized peptide derived from human NFAT5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NFAT5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 1D6
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- Nuclear Factor Of Activated T Cells 5; Nuclear Factor Of Activated T-Cells 5; Nuclear Factor Of Activated T-Cells 5, Tonicity-Responsive; Tonicity-Responsive Enhancer-Binding Protein; T-Cell Transcription Factor NFAT5; TonE-Binding Protein; NF-AT5; TONEBP; Tonicity-Responsive Enhancer Binding Protein; Osmotic Response Element-Binding Protein
- Gene ID
- 10725
- UniProt ID
- O94916
- Long Name
- Nuclear Factor Of Activated T Cells 5
- Cellular Localization
- Nucleus, Chromosome, Cytoplasm
- Post Translation Modifications
- Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization.
- Protein Refseq
- NP_001106649.1
- Function
- Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'.
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "NFAT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2815z | Mouse Anti-NFAT5 Recombinant Antibody (clone 40B8) | WB, ELISA, IF, IP | Mouse IgG2a, κ |
| MOB-1707MZ | Recombinant Mouse Anti-Human NFAT5 Antibody (clone 1H16) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1487 | Anti-NFAT5 Immunohistochemistry Kit | IHC |
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