Mouse Anti-NHP ALOX15 Recombinant Antibody (clone OTI3G8)
CAT#: VS-1024-XY21
This monoclonal antibody is derived from a mouse immunized with full-length human recombinant ALOX15 protein. It is highly specific for ALOX15 and shows strong reactivity with human, non-human primate, and rat tissues. The antibody is validated for use in multiple applications, including Western Blot (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC), providing researchers with a versatile tool for investigating ALOX15 expression and function across different species and experimental contexts.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human bronchus
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13859/30915_B_4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human spleen showsstrong cytoplasmic positivity in a subset of cells in red pulp.
Immunohistochemical staining of human spleen showsstrong cytoplasmic positivity in a subset of cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4962/16100_B_7_4_selected.jpg
Normal Tissue
Figure 3 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4963/16092_B_8_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13859/30915_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13859/30915_B_9_5.jpg
Normal Tissue
Figure 6 Nasopharynx
Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13859/30915_B_7_2.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13859/30915_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13859/30915_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13859/30915_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Group enriched (HMC-1, MCF7, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161905-ALOX15
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Specifications
- Immunogen
- Full length human recombinant protein of ALOX15
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- ALOX15
- Species Reactivity
- Human, Non-human primate, Rat
- Clone
- OTI3G8
- Applications
- WB, IHC, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, ICC: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
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- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ALOX15"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1155CT | Mouse Anti-ALOX15 Recombinant Antibody (clone EML159) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC47 | CytoStream™ Rabbit Anti-ALOX15 Recombinant Antibody (VS13-YC47) | IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY323 | Anti-ALOX15 Immunohistochemistry Kit | IHC | |
| VS-0525-XY324 | Anti-Human ALOX15 Immunohistochemistry Kit | IHC | |
| VS-0525-XY325 | Anti-Rat ALOX15 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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