Mouse Anti-NHP GCLC Recombinant Antibody (clone OTI1A3)
CAT#: VS-1024-XY217
Designed to target GCLC, this mouse monoclonal antibody demonstrates reactivity with human, mouse, non-human primate, and rat tissues. The immunogen is a full-length recombinant human GCLC protein, ensuring precise specificity. It excels in Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications, providing reliable performance for comprehensive studies of GCLC expression and function across various experimental models.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36360/432_C2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9569/931_H5_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36359/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36359/84384_A_5_6.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9569/24577_A_9_8.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36359/84384_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36359/84384_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, Hep G2, OE19, RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000001084-GCLC
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Specifications
- Immunogen
- Full length human recombinant protein of GCLC
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- GCLC
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- OTI1A3
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:200-1:800, IHC: 1:200, IF: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1995MZ | Recombinant Mouse Anti-Human GCLC Antibody | ICC, IF, WB | Mouse antibody |
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| VS3-FY583 | Recombinant Rabbit Anti-GCLC Antibody (clone R03-9D9) | WB | Rabbit IgG |
| VS3-FY584 | Recombinant Rabbit Anti-GCLC Antibody (clone R08-2F5) | WB, IP | Rabbit IgG |
| VS3-FY2718 | Rabbit Anti-GCLC Recombinant Antibody (VS3-FY2718) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1417 | Hi-Affi™ Rabbit Anti-GCLC Recombinant Antibody (clone DS1417AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2767 | Anti-Rat GCLC Immunohistochemistry Kit | IHC |
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