Rabbit Anti-P. falciparum MSP1 Recombinant Antibody (clone 5.2) (CAT#: FAMAB-1485CQ)

This product is a recombinant rabbit antibody that recognizes P. falciparum MSP1. The antibody was purified by affinity chromatography. It can be used in IB, IP, ELISA, IF.


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IF

Figure 1 Indirect immunofluorescence assay.

Figure 1 Indirect immunofluorescence assay.

Indirect immunofluorescence assay of acetone-fixed P. falciparum schizonts stained with antibody 5.2 to the parasite surface (a) and antibody 219.5 to the rhoptry organelles (b).

Siddiqui, W. A., Tam, L. Q., Kan, S. C., Kramer, K. J., Case, S. E., Palmer, K. L., ... & Hui, G. S. (1986). Induction of protective immunity to monoclonal-antibody-defined Plasmodium falciparum antigens requires strong adjuvant in Aotus monkeys. Infection and immunity, 52(1), 314-318.

Figure 2 Antibodies 5.2 and 219.5 were found to coidentify with immune Aotus IgG five prominent and other minor metabolically labeled polypeptides.

Figure 2 Antibodies 5.2 and 219.5 were found to coidentify with immune Aotus IgG five prominent and other minor metabolically labeled polypeptides.

Coidentification of [3H]isoleucine-labeled P. falciparum polypeptides by monoclonal antibody 5.2 to the parasite surface and monoclonal antibody 219.5 to the parasite rhoptry organelles with polypeptides bound by immune Aotus IgG. The metabolically labeled polypeptides were bound to antibody 5.2 (lane 1) or antibody 219.5 (lane 3) immunoabsorbents, eluted, and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a 6.25% gel. The unbound polypeptides passing through the monoclonal antibody 5.2 and 219.5 immunoabsorbents were then adsorbed to immune Aotus IgG immunoabsorbent, eluted, and analyzed (lanes 2 and 4, respectively). Total polypeptides bound by immune Aotus IgG are shown in lane 5.

Siddiqui, W. A., Tam, L. Q., Kan, S. C., Kramer, K. J., Case, S. E., Palmer, K. L., ... & Hui, G. S. (1986). Induction of protective immunity to monoclonal-antibody-defined Plasmodium falciparum antigens requires strong adjuvant in Aotus monkeys. Infection and immunity, 52(1), 314-318.


Specifications

  • Immunogen
  • P. falciparum
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Native Plasmodium falciparum merezoite surface protein 1
  • Species Reactivity
  • P. falciparum
  • Clone
  • 5.2
  • Applications
  • IB, IP, ELISA, IF

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • MSP-1; Merozoite surface protein 1; P. falciparum MSP-1; Plasmodium falciparum

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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