CytoStream™ Mouse Anti-PDIA4 Recombinant Antibody (VS13-YC887)
CAT#: VS13-YC887
The CytoStream™ Mouse Anti-PDIA4 Recombinant Antibody is specific for human, mouse and rat PDIA4. PDIA4 encodes an ER protein enhancing antibody assembly through disulfide bonding, interacting with proteins like cyclophilin B, possessing spliced variants. The anti-PDIA4 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17368/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6139/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17368/ihc_selected.jpg
Normal Tissue
Figure 4 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_A_1_7.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_B_7_5.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_A_8_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155660-PDIA4
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Specifications
- Immunogen
- Recombinant human ERp72, aa 596-645.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Rat PDIA4
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 50% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Protein Disulfide Isomerase Family A Member 4; Endoplasmic Reticulum Resident Protein 70; Endoplasmic Reticulum Resident Protein 72; Protein Disulfide Isomerase-Associated 4; ER Protein 70; ER Protein 72; EC 5.3.4.1; ERp-72;
- Gene ID
- 9601
- UniProt ID
- P13667
- Long Name
- Protein Disulfide Isomerase Family A Member 4
- Cellular Localization
- Endoplasmic reticulum
- Function
- Signal transduction
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PDIA4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2830z | Mouse Anti-PDIA4 Recombinant Antibody (clone 8C9) | WB, ICC, IF | Mouse IgG1 |
| MOB-0700CT | Recombinant Mouse anti-Human PDIA4 Monoclonal antibody (EML1008) | WB | |
| MRO-0552-CN | Recombinant Mouse Anti-PDIA4 Monoclonal Antibody (10-F2) | WB, IHC, IF, FC | Mouse IgG |
| MRO-1847-CN | Rabbit Anti-PDIA4 Polyclonal Antibody (MRO-1847-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-FY1119 | Recombinant Rabbit Anti-PDIA4 Antibody (clone R02-1J3) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4359 | Hi-Affi™ Recombinant Rabbit Anti-PDIA4 Monoclonal Antibody (SI449DS) | ICC, IF, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC668 | AbPlus™ Anti-PDIA4 Magnetic Beads (VS-0724-YC668) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY192 | Mouse Anti-NHP PDIA4 Recombinant Antibody (clone OTI2B9) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5287 | Anti-PDIA4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5288 | Anti-Mouse PDIA4 Immunohistochemistry Kit | IHC |
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