Recombinant Rabbit Anti-PDIA4 Antibody (clone R02-1J3) (CAT#: VS3-FY1119)
This product is a recombinant rabbit antibody that recognizes PDIA4. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R02-1J3 is specific for human PDIA4. The antigen is synthetic peptide of human erp72.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17368/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17368/if_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6139/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6139/ihc_selected.jpg
Figure 3 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17368/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17368/ihc_selected.jpg
Figure 4 Placenta
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_A_1_7.jpg
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_A_1_7.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_B_7_5.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6140/16418_A_8_4.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155660-PDIA4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155660-PDIA4
Specifications
- Immunogen
- Synthetic peptide of human ERp72.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PDIA4
- Species Reactivity
- Human
- Clone
- R02-1J3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 73 kDa; Observed MW: 73 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Protein Disulfide Isomerase Family A Member 4; Endoplasmic Reticulum Resident Protein 70; Endoplasmic Reticulum Resident Protein 72; Protein Disulfide Isomerase-Associated 4; ER Protein 70; ER Protein 72; EC 5.3.4.1; ERp-72
- Gene ID
- 9601
- UniProt ID
- P13667
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2830z | Mouse Anti-PDIA4 Recombinant Antibody (clone 8C9) | WB, ICC, IF | Mouse IgG1 |
| MOB-0700CT | Recombinant Mouse anti-Human PDIA4 Monoclonal antibody (EML1008) | WB | |
| MRO-0552-CN | Recombinant Mouse Anti-PDIA4 Monoclonal Antibody (10-F2) | WB, IHC, IF, FC | Mouse IgG |
| MRO-1847-CN | Rabbit Anti-PDIA4 Polyclonal Antibody (MRO-1847-CN) | WB, IF, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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