Anti-PHGDH Immunohistochemistry Kit
CAT#: VS-0325-XY1642
Our PHGDH IHC Kit features a full range of reagents, simplifying the reagent gathering process. It is designed for easy handling, making it suitable for laboratories. Compatible with both paraffin and frozen tissue sections, it enables reliable detection of PHGDH, meeting the needs of research projects on PHGDH - related metabolic pathways.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21241/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24031/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cervix, uterine
Immunohistochemical staining of human cervix, uterine shows cytoplasmic and nuclear positivity in squamous epithelia.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21241/158736_B_7_3_selected.jpg
Normal Tissue
Figure 4 Parathyroid gland.
High expression in parathyroid gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3681/10161_A_1_8_val_selected.jpg
Normal Tissue
Figure 5 IHC staining of human skin
Immunohistochemical staining of human skin shows strong cytoplasmic and nuclear positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68216/ihc_selected.jpg
Normal Tissue
Figure 6 Salivary gland
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68216/152505_B_3_1.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68216/152505_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68216/152505_A_8_5.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68216/152505_A_7_8.jpg
Normal Tissue
Figure 10 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68216/152505_B_9_5.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000092621-PHGDH
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- PHGDH
- Primary Antibody
- Mouse Anti-PHGDH Monoclonal Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PHGDH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2201z | Mouse Anti-PHGDH Recombinant Antibody (clone 29H3) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC510 | AbPlus™ Anti-PHGDH Magnetic Beads (VS-0724-YC510) | IP, Protein Purification | |
| VS-0724-YC907 | AbPlus™ Anti-PHGDH Magnetic Beads (VS-0724-YC907) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5371 | Anti-Mouse PHGDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY5370 | Anti-Human PHGDH Immunohistochemistry Kit | IHC |
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