Mouse Anti-PKM Recombinant Antibody (VS3-CJ43)
CAT#: VS3-CJ43
This product is a mouse antibody that recognizes PKM.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/283_B10_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067225-PKM
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Specifications
- Immunogen
- Recombinant fragment of human PKM2 (N-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PKM
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
- Gene ID
- 5315
- UniProt ID
- P14618
- Sequence Similarities
- Belongs to the pyruvate kinase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- ISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
Isoform M2:
Acetylated at Lys-433 by EP300, leading to impair phosphoenolpyruvate substrate-binding and promote its homodimerization and subsequent translocation to the nucleus (PubMed:24120661).
Deacetylation at Lys-433 by SIRT6 promotes its nuclear export into the cytoplasm, leading to suppress its nuclear localization and oncogenic function (PubMed:26787900).
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
- Protein Refseq
- NP_001193725.1; NP_001193726.1; NP_001193727.1
- Function
- Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (PubMed:20847263, PubMed:15996096, PubMed:1854723).
The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production (PubMed:20847263, PubMed:15996096, PubMed:1854723).
The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival (PubMed:20847263, PubMed:15996096, PubMed:1854723).
Isoform M2:
Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity (PubMed:18337823, PubMed:20847263).
In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase (PubMed:18191611, PubMed:21620138, PubMed:22056988, PubMed:22306293, PubMed:22901803, PubMed:24120661).
Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase (PubMed:22056988, PubMed:22306293, PubMed:22901803, PubMed:24120661, PubMed:26787900).
Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription (PubMed:22306293, PubMed:22901803, PubMed:24120661).
Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis (PubMed:18337823, PubMed:22901803, PubMed:26787900).
Promotes the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (By similarity).
May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs (By similarity).
Plays a role in caspase independent cell death of tumor cells (PubMed:17308100).
Isoform M1:
Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth (PubMed:18337823).
In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity (PubMed:20847263).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PKM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0378C | Mouse Anti-PKM Recombinant Antibody (ZG-0378C) | WB, ICC | Mouse IgG |
| ZG-0379C | Mouse Anti-PKM Recombinant Antibody (ZG-0379C) | WB, ICC | Mouse IgG |
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| VS3-QX880 | Mouse Anti-PKM Recombinant Antibody (clone 1A7-G6-H6) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY393 | Rabbit Anti-NHP PKM Recombinant Antibody (VS-1024-XY393) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY178 | CytoStream™ Rabbit Anti-PKM Recombinant Antibody (VS-0225-XY178) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5443 | Anti-PKM Immunohistochemistry Kit | IHC | |
| VS-0525-XY5444 | Anti-Rat PKM Immunohistochemistry Kit | IHC | |
| VS-0525-XY5445 | Anti-Mouse PKM Immunohistochemistry Kit | IHC |
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