Mouse Anti-PNA-DNA duplex Recombinant Antibody (clone MHH5)
CAT#: FAMAB-0251YC
PNA peptide nucleic acid molecules are newly developed DNA analogues with the capacity to bind nucleic acids in a sequence specific manner with very high affinity. PNA/DNA duplexes are formed by Watson–Crick or Hoogsteen basepairing, and it is believed
that they form a helical structure that is not very different from that of DNA/DNA.The MHH5 clone was tested for reactivity with a variety of PNA/DNA duplexes in ELISA.
Published Data
ELISA
Figure 1 Specificity of MHH5 Fab-phage clone.
Reactivity with single- and double-stranded DNA and PNA molecules was analyzed by immobilization of various duplexes to streptavidin coated microtiter wells, followed by addition of increasing numbers of Fab-phages/well.
Haahr Hansen, M. H., Sode, L. L., Hyldig-Nielsen, J. J., & Engberg, J. (1997). Detection of PNA/DNA hybrid molecules by antibody Fab fragments isolated from a phage display library. Journal of immunological methods, 203(2), 199–207.
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Specifications
- Immunogen
- H12 duplex
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- PNA-DNA duplex
- Clone
- MHH5
- Applications
- ELISA, DB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Peptide nucleic acid/DNA duplex; PNA/DNA duplex
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251-YC-S(P) | Mouse Anti-PNA-DNA duplex Recombinant Antibody (clone MHH5); scFv Fragment | ELISA, DB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251-YC-F(E) | Mouse Anti-PNA-DNA duplex Recombinant Antibody (clone MHH5); Fab Fragment | ELISA, DB | Mouse Fab |
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