Mouse Anti-PPARA Recombinant Antibody (clone 5D10C3) (CAT#: VS7-0425-WR655)
This product is a mouse IgG1 antibody that binds to human PPARA. The antibody 5D10C3 recognizes specific amino acid domain of human PPARA (AA: 1-120). It can be used for WB and FC assays. It cross reacts with monkey.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58901/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58901/if_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_5.jpg
Figure 7 Testis
(Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_6_6.jpg
(Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186951-PPARA/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186951-PPARA/subcellular
Specifications
- Immunogen
- Recombinant human PPARA (AA: 1-120) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PPARA
- Species Reactivity
- Human, Monkey
- Clone
- 5D10C3
- Molecule Class
- Peroxisome Proliferator-Activated Receptor (Ppar) Nuclear Receptor
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PPARA; Peroxisome Proliferator-Activated Receptor Alpha; PPAR; PPAR-Alpha; HPPAR; Peroxisome Proliferative Activated Receptor, Alpha; Peroxisome Proliferator-Activated Nuclear Receptor Alpha Variant 3
- Gene ID
- 5465
- UniProt ID
- Q07869
- Long Name
- Peroxisome proliferator activated receptor alpha
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubl conjugation
- Protein Refseq
- NP_001001928.1
- Function
- Activator; DNA-binding; Receptor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
PPAR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Chicken IgY Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3743z | Mouse Anti-PPARA Recombinant Antibody (clone 42H1) | WB, ICC, IF, FC, IP, FuncS | Mouse IgG2b |
| MOB-1489CT | Recombinant Mouse anti-Human PPARA Monoclonal antibody (I0834) | ChIP, ELISA, EMSA, IP, WB | |
| VS3-QX896 | Mouse Anti-PPARA Recombinant Antibody (clone 1331CT894.186.143) | WB, ICC, IHC, FC | Mouse IgG1, κ |
| VS3-WK895 | Mouse Anti-PPARA Recombinant Antibody (VS3-WK895) | WB, FC, IF, IHC-P | Mouse IgG1, κ |
| VS7-0425-WR654 | Mouse Anti-PPARA Recombinant Antibody (clone 5D10E10) | FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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